RT Journal Article
T1 A serum cytotoxicity assay for measuring the resistance of turbot to the ciliate Philasterides dicentrarchi
A1 Gulías Otero, Paola
A1 Sueiro Benavides, Rosa Ana
A1 González Barreiro, Óscar
A1 Brea Portela, Cristina
A1 Macías Sancho, Joaquín
A1 Leiro Vidal, José Manuel
A1 Lamas Fernández, Jesús
K1 Philasterides dicentrarchi
K1 Fish
K1 Complement system
K1 Cytotoxicity
K1 Immunization
K1 Disease resistance
AB In previous studies we have found that the complement system plays an essential role in the resistance of fish to infection by the ciliate parasite Philasterides dicentrarchi. The present study shows that it is possible to predict resistance to P. dicentrarchi in the turbot Scophthalmus maximus by using a complement-dependent serum cytotoxicity assay. Fish were immunised against P. dicentrarchi, and fish mortality following experimental challenge, specific serum antibody levels and serum cytotoxicity were compared. We found a correlation between the results obtained regarding the killing activity of serum and the protection generated in turbot by the vaccine after experimental challenge. The study findings suggest that the serum cytotoxicity assay can be used to assess the protection generated by the vaccine against P. dicentrarchi in turbot, thereby reducing both the number of fish required for the experiments and the pain and distress associated with experimental infection
PB Elsevier
SN 2950-3116
YR 2025
FD 2025-10-30
LK https://hdl.handle.net/10347/44491
UL https://hdl.handle.net/10347/44491
LA eng
NO Guliás P, Sueiro RA, González O, Brea C, Macías J, Leiro JM, Lamas J. (2025). A serum cytotoxicity assay for measuring the resistance of turbot to the ciliate Philasterides dicentrarchi. Comparative Immunology Reports 9 (2025), 200259. doi: https://doi.org/10.1016/j.cirep.2025.200259
NO IDI-20200702 from the Ministerio de Economía, Industria y Competitividad
NO 2021-PG042 from the Xunta de Galicia (Spain)
NO Programa de Ciencias Mariñas de Galicia (2022-CP084)
DS Minerva
RD 23 abr 2026