RT Journal Article
T1 Validation of a simple sample preparation method for multielement analysis of bovine serum
A1 Luna Narváez, Diego Fernando
A1 Miranda Castañón, Marta Inés
A1 Hamad Minervino, Antonio Humberto
A1 Piñeiro Gómez, Verónica
A1 Herrero Latorre, Carlos
A1 López Alonso, María Marta
K1 Trace elements
K1 Chemical elements
K1 Blood
K1 Cattle
K1 Brain damage
K1 Hydrogen peroxide
AB Here we propose a single acid digestion (SAD) sample preparation method for ICP-MSanalysis of animal serum samples to determine trace element contents. The method wasevaluated in comparison with a commonly used procedure involving dilution of samples inan alkaline solution (AKD). In the SAD procedure, aliquots (1 mL) of bovine serum sampleswere treated at low temperature with a mixture of concentrated nitric acid and hydrogen peroxide. Trace elements (As, B, Ba, Cd, Co, Cr, Cu, Fe, Hg, Li, Mn, Mo, Ni, Pb, Sb, Se, Sr, U,and Zn) were directly determined by ICP-MS analysis of diluted solutions of samples. Bothmethods were sufficiently sensitive to enable quantification of most trace elements, with theexception of the AKD method for Cd, Hg and Pb. The quality of the data was verified byusing certified reference material. Good results were obtained for the SAD procedure and allelements, but recoveries were unacceptable with the AKD procedure for Se (recovery:57%), Cd (154%) and Fe (139%). Strong associations (R2>0.90, P = 0.000) between thedata obtained by both methods were demonstrated for the elements considered. The proposed SAD sample preparation method produced satisfactory results for determining mosttoxic and essential trace elements targeted in monitoring studies.
PB PLOS
YR 2019
FD 2019
LK http://hdl.handle.net/10347/20977
UL http://hdl.handle.net/10347/20977
LA eng
NO Luna, D., Miranda, M., Minervino, A. H. H., Pineiro, V., Herrero-Latorre, C., & Lopez-Alonso, M. (2019). Validation of a simple sample preparation method for multielement analysis of bovine serum. PloS one, 14(2).
DS Minerva
RD 24 abr 2026