RT Journal Article
T1 Molecular epidemiology of photobacterium damselae subsp. damselae outbreaks in marine rainbow trout farms reveals extensive horizontal gene transfer and high genetic diversity
A1 Terceti, Mateus de Souza
A1 Vences Lorenzo, Ana
A1 Matanza Fente, José Manuel
A1 Dalsgaard, Inger
A1 Pedersen, Karl
A1 Rodríguez Osorio, Carlos
K1 Photobacterium damselae
K1 Vibriosis
K1 Damselysin
K1 Phobalysin
K1 Hemolysin
K1 Rainbow trout
AB The marine bacterium Photobacterium damselae subsp. damselae is a pathogen for a variety of marine animals, as well as for humans, and is nowadays considered an emerging pathogen for fish of importance in marine aquaculture. Recent studies have suggested that outbreaks in fish farms are caused by multiclonal populations of this subspecies that exist in the environment. Here, we report the study of a collection of 31 strains isolated during the course of disease outbreaks in marine rainbow trout farms in Denmark in 1994, 1995, and 2006, respectively. A phylogenetic analysis based on the toxR gene sequence, and the screening of virulence-related genes uncovered a high genetic heterogeneity, even among strains isolated from the same fish farm at the same time. Moreover, comparative analysis of the whole genome sequences of four selected strains revealed a large number of differentially occurring genes, which included virulence genes, pPHDD1 plasmid, polysaccharide synthesis gene clusters, CRISPR-Cas systems and putative new mobile genetic elements. This study provides sound evidence that P. damselae subsp. damselae outbreaks in Danish rainbow trout farms were caused by multiclonal populations and that horizontal gene transfer constitutes a strong driving force in the generation of intraspecific diversity in this pathogen
PB Frontiers Media
YR 2018
FD 2018
LK http://hdl.handle.net/10347/22698
UL http://hdl.handle.net/10347/22698
LA eng
NO Terceti MS, Vences A, Matanza XM, Dalsgaard I, Pedersen K and Osorio CR (2018) Molecular Epidemiology of Photobacterium damselae subsp. damselae Outbreaks in Marine Rainbow Trout Farms Reveals Extensive Horizontal Gene Transfer and High Genetic Diversity. Front. Microbiol. 9:2155. doi: 10.3389/fmicb.2018.02155
NO This work has been supported by grant AGL2016-79738-R(AEI/FEDER, EU) from the State Agency for Research (AEI)of Spain, and co-funded by the FEDER Programme from theEuropean Union. The support of Xunta de Galicia (Spain)with grant GRC-2014/007 is also acknowledged. MT thanks theBrazilian Ministry of Education and CAPES (Coordenaçao deAperfeiçoamento de Pessoal de Nível Superior) for a predoctoralfellowship. XM thanks Xunta de Galicia for a predoctoralfellowship
DS Minerva
RD 29 abr 2026