RT Journal Article
T1 Genetic markers associated with divergent selection against the parasite Marteilia cochillia in common cockle (Cerastoderma edule) using transcriptomics and population genomics data
A1 Pampín Iglesias, Marina
A1 Fernández López, José Carlos
A1 Blanco Hortas, Andrés
A1 Hermida Prieto, Miguel
A1 Vera Rodríguez, Manuel
A1 Gómez Pardo, María Belén
A1 Coimbra, Maria Raquel Moura
A1 Cao Hermida, María Asunción
A1 Iglesias Estepa, David
A1 Carballal Durán, María Jesús
A1 Villalba García, Antonio
A1 Martínez Portela, Paulino
A1 Casanova Chiclana, Adrián
K1 SNP
K1 Bivalve
K1 Cockles
K1 Transcriptomics
K1 Population genomics
K1 Marteilia cochillia
K1 Resilience
AB The common cockle (Cerastoderma edule) plays an important role in marine ecosystems and represents a valuable socioeconomic resource for coastal communities. In 2012, the cockle beds from Rı́a de Arousa (Galicia, NW Spain) were seriously decimated by the protozoan Marteilia cochillia responsible for marteiliosis. We aimed to identify single nucleotide polymorphisms (SNP) markers potentially associated with resilience to marteiliosis to be used in marker-assisted selection programs for restoring affected cockle beds and recovering their production. For this, we carried out a population genomics approach using 2b-RADseq, where 38 naive samples (before the first detection of M. cochillia in 2012) from two beds of Rı́a de Arousa were compared with 39 affected samples collected in 2018/2019 (after several years of marteiliosis occurring in the area), collected either before (15 non-exposed samples) or during (24 exposed samples) the marteiliosis outbreak. Additionally, 767 differentially expressed genes (DEG) from a previous transcriptomic study addressed during the aforementioned 2018/19 marteiliosis outbreak, were evaluated to identify SNPs showing signals of selection. Using 2b-RADseq, 9,154 SNPs were genotyped and among them, 110 consistent outliers for divergent selection were identified. This set of SNPs was able to discriminate the samples according to their marteiliosis status (naive vs affected; exposed vs non-exposed), while another 123 SNPs were identified linked to DEGs associated with the level of infection across a temporal series. Finally, combining the population genomics and transcriptomics information, we selected the 60 most reliable SNPs associated with marteiliosis resilience. These SNPs were close to or within DEGs, and many of them were related to immune response (phagocytosis and cell adhesion), defence, such as apoptosis, stress, and cellular cycle, among other functions. This set of SNPs will eventually be validated to develop a cost-effective genotyping tool for their application for obtaining cockle-resilient strains for marteiliosis.
PB Frontiers media
YR 2023
FD 2023
LK http://hdl.handle.net/10347/32456
UL http://hdl.handle.net/10347/32456
LA eng
NO Pampín, M., Casanova, A., Fernández, C., Blanco, A., Hermida, M., Vera, M., Pardo, B. G., Coimbra, R. M., Cao, A., Iglesias, D., Carballal, M. J., Villalba, A. and Martínez, P. (2023). Genetic markers associated with divergent selection against the parasite Marteilia cochillia in common cockle (Cerastoderma edule) using transcriptomics and population genomics data. Frontiers in Marine Science, 10. 1-14. https://doi.org/10.3389/fmars.2023.1057206
NO This study was possible due to funds granted by the EuropeanUnion through the project COCKLES within the INTERREG-AAprogramme (EAPA_458/2016) and the scholarship granted toM.R.M.Coimbra from CNPq (202015/2020-3). This research hasbeen carried out under the framework of the Spain's Recovery andResilience Plan, and more specifically under the investment lineno.1 of its component number 17, where the complementary RTDI plan with the autonomous regions of Spain are foreseen, with one ofthose being the Complementary RTDI Plan for Marine Sciencewhich includes the Marine Science Programme for Galicia. Thisresearch in this paper corresponds to the Programme WorkPackage nº 6 and activity no. 6.3.A.2 about "Genetic architectureof marteiliosis resistance in common cockle" and has been fundedby the Resilience and Recovery Funds).
DS Minerva
RD 24 abr 2026