RT Journal Article T1 Potential of endogenous PHA as electron donor for denitrification A1 Santorio Aldariz, Sergio A1 Fra Vázquez, Andrea A1 Val del Río, Ángeles A1 Mosquera Corral, Anuska K1 Activity test K1 Denitrification K1 Polyhydroxyalkanoates K1 Specific endogenous denitrifying activity AB The use of wastewater streams to obtain polyhydroxyalkanoates (PHA) as high added-value products is widely studied. However, nitrogen removal is not well integrated into this process. In this study, the optimal conditions to track the specific endogenous denitrifying activity (SEDA) driven by PHA as carbon source were selected as: sludge concentration of 0.5–2 g VSS/L, CODPHA/N ratio higher than 5.4 g/g and between 40 and 60 mg NO3−-N/L. The seeding biomass used to perform the activity tests was collected from two sequencing batch reactors and was able to store up to 69% wt/wt of PHA. SEDA values of 0.26–0.39 g N2-N/(g VSSact d) were achieved, which proved the potential of PHA-accumulating mixed microbial cultures to be used in nitrogen removal processes. The results indicated that there is not a preference in the consumption of hydroxybutyrate over hydroxyvalerate and that PHA concentrations lower than 5% wt/wt do not allow the obtainment of the maximum SEDA value. Finally, N2O gas production was not detected in the SEDA experiments PB Elsevier SN 0048-9697 YR 2019 FD 2019 LK http://hdl.handle.net/10347/25149 UL http://hdl.handle.net/10347/25149 LA eng NO Science of The Total Environment, 695 (2019), 133747. https://doi.org/10.1016/j.scitotenv.2019.133747 NO The authors would like to thank the Spanish Government (AEI) for funding in the frame of the projects TREASURE (CTQ2017-83225-C2-1-R) and AQUAVAL (PCIN-2017-047), this last also funded by EU in the frame of the collaborative international Consortium AQUAVAL financed under the ERA-NET WaterWorks2015 Cofunded Call. This ERA-NET is an integral part of the 2016 Joint Activities developed by the Water Challenges for a Changing World Joint Programme Initiative (Water JPI). The authors belong to the Galician Competitive Research Group (GRC-ED431C 2017-29) and to the CRETUS Strategic Partnership (ED431E 2018/01), co-funded by FEDER (UE). Angeles Val del Rio is a Xunta de Galicia fellow (ED418B 2017/075) DS Minerva RD 29 abr 2026