RT Journal Article
T1 Tuna skin as bioresource for gelatine: Extraction with ionic liquid
A1 Souto Moreno, Paula
A1 Gallego Rodríguez, Cristina
A1 Vázquez Álvarez, José Antonio
A1 Rodil Rodríguez, Eva
A1 Soto Campos, Ana María
K1 Gelatine
K1 Fish skin
K1 Maceration
K1 Ionic liquid
AB Unlocking the ocean’s full potential requires converting fish waste into materials and products of interest, thus improving the circularity and sustainability of the fish processing industry. Fish skin is an excellent bioresource for obtaining gelatine, a product of high interest in many industries due to its multifunctionality. The current extraction methods involve several stages with harsh chemicals, which pose environmental concerns. In this work, an innovative method consisting of maceration with ionic liquid and extraction with water is proposed. Unlike other methods based on ionic liquids or deep eutectic solvents that focus on dissolving the fish by-product, and therefore require an energy-intensive stage to recover the protein, a non-dissolving treatment is proposed to facilitate protein extraction with water. The gelatine is precipitated from the aqueous solution by adding acetone. The method was tested obtaining gelatine from tuna skin and using 1-ethyl-3-methylimidazolium acetate, a low toxicity ionic liquid able to disrupt the skin through hydrogen bonding. Optimised conditions led to a recovery yield of 0.188 g gelatine/g wet skin. FTIR showed the characteristic bands corresponding to molecular vibrations and functional groups of the gelatine structure. α- and β-chains constitute approximately the 43 % of the product, with the remainder being peptides with an average molecular weight of 44 kDa. The pyrrolidine amino acids content was 21 %. The gelatine showed good thermal stability. Melting and gelling temperature (20.6 and 12.9 °C, respectively) fall within the typical range for warm water fish. As an advantage in comparison to classical methods, the proposed method avoids the need of multiple treatment steps with strong acids and alkalis.
PB Elsevier
SN 1383-5866
YR 2025
FD 2025-08-14
LK https://hdl.handle.net/10347/43299
UL https://hdl.handle.net/10347/43299
LA eng
NO Souto-Montero, P., Gallego, C., Vázquez, J. A., Rodil, E., & Soto, A. (2025). Tuna skin as bioresource for gelatine: Extraction with ionic liquid. Separation and Purification Technology, 363, 132144. 10.1016/j.seppur.2025.132144
NO The authors are grateful to Rosario Rodil from the Institute of Research on Chemical and Biological Analysis (IAQBUS) of the Universidade de Santiago de Compostela, for his help with the design of experiments. The authors also thank Jealsa Foods S.A.U. (Boiro, Galicia, Spain) for the kind supply of fish skin for the experiments.
NO Grant PID2021-123622OB-I00 funded by MCIN/AEI/10.13039/ 501100011033 and by “ERDF A way of making Europe” by the European Union. Author from IIM-CSIC is grateful to Xunta de Galicia (GPC_IN607B 2024/010) for funding.
DS Minerva
RD 23 abr 2026