RT Journal Article
T1 Development and validation of a liquid chromatography tandem mass spectrometry method for the determination of cannabinoids and phase I and II metabolites in meconium
A1 Prego-Meleiro, Pablo
A1 Lendoiro Belío, Elena
A1 Concheiro, Marta
A1 Cruz Landeira, Angelines
A1 López-Rivadulla Lamas, Manuel
A1 Castro Ríos, Ana de
K1 Cannabis
K1 Metabolite
K1 Glucuronide
K1 Meconium
K1 LC–MS/MS
AB A liquid chromatography–tandem mass spectrometry (LC–MSMS) method was developed and fully validated for the determination of Δ9-tetrahydrocannabinol (THC), 11-hydroxyTHC (OHTHC), 11-nor-9-carboxyTHC (THCCOOH), 8-β-11-dihydroxyTHC (diOHTHC), cannabinol, cannabidiol, and THC and THCCOOH glucuronides in 0.25 ± 0.02 g meconium. Samples were homogenized in methanol and subjected to cation exchange solid-phase extraction. Chromatographic separation was performed on a Kinetex C18 column (50 mm × 2.1 mm, 2.6 μm) at 35 °C, with a gradient of 0.1% formic acid in water and acetonitrile at a flow rate of 0.3 mL/min; total run time was 10 min. Two transitions per analyte were monitored in MRM mode. The method was specific and sensitive; LOD was from 1 to 2 ng/g, and LOQ from 4 to 10 ng/g; linearity ranged from 4 to 400 ng/g for all the analytes, except for THC glucuronide (10–400 ng/g); intra-assay, inter-assay and total imprecision were <11.2%, <13.45% and <15.6%, respectively; accuracy ranged from 93.9% to 109.0% of the target concentration; matrix effect, extraction and process efficiency ranged from −26.4% to −71.4%, 49.9% to 69.5% and 14.3% to 45.0%, respectively. The inclusion of THC and THCCOOH glucuronides avoided the need for the hydrolysis process, thus facilitating sample pretreatment. Application of the method to 19 authentic meconium specimens from uncontrolled pregnancies or women suspicious of drug consumption revealed fetal cannabis exposure in 4 newborns. THCCOOH (24.1–288.8 ng/g), diOHTHC (53.2–332.4 ng/g), THC (4.2–7.7 ng/g), CBN (30.7–93.3 ng/g) and CBD (7.1–251.5 ng/g) were detected in all cases; THCCOOH glucuronide (190.2–306.8 ng/g) in 3 cases; and OHTHC (11.9 ng/g) in the remaining one; however, THC glucuronide was not identified in any specimen.
PB Elsevier B.V.
SN 1873-3778
YR 2017
FD 2017
LK https://hdl.handle.net/10347/38861
UL https://hdl.handle.net/10347/38861
LA eng
NO Pablo Prego-Meleiro; Elena Lendoiro; Marta Concheiro; Angelines Cruz; Manuel López-Rivadulla; Ana de Castro. Development and validation of a liquid chromatography tandem mass spectrometry method for the determination of cannabinoids and phase I and II metabolites in meconium. J Chromatogr A. 2017;1497:118-126
NO This study was supported by the Plan Nacional de Drogas, Ministerio de Sanidad, Gobierno de España (2016I005 Project). E. Lendoiro and A. de Castro would like to thank the Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia, for their predoctoral and postdoctoral contracts, respectively (PRE/2011/072 and POS-B/2013/003).
DS Minerva
RD 30 abr 2026