RT Journal Article
T1 An elastin-like recombinamer-based bioactive hydrogel embedded with mesenchymal stromal cells as an injectable scaffold for osteochondral repair
A1 Cipriani, Filippo
A1 Ariño Palao, Blanca
A1 González de Torre, Israel
A1 Vega Castrillo, Aurelio
A1 Aguado Hernández, Héctor José
A1 Alonso Rodrigo, Matilde
A1 Álvarez Barcia, Ángel José
A1 Sánchez, Ana
A1 García Diaz, Verónica
A1 López Peña, Mónica
A1 Rodríguez Cabello, José Carlos
K1 Biopolymer
K1 Animal test
K1 Cartilage
K1 Bone
AB The aim of this study was to evaluate injectable, in situ cross-linkable elastin-like recombinamers (ELRs) for osteochondral repair. Both the ELR-based hydrogel alone and the ELR-based hydrogel embedded with rabbit mesenchymal stromal cells (rMSCs) were tested for the regeneration of critical subchondral defects in 10 New Zealand rabbits. Thus, cylindrical osteochondral defects were filled with an aqueous solution of ELRs and the animals sacrificed at 4 months for histological and gross evaluation of features of biomaterial performance, including integration, cellular infiltration, surrounding matrix quality and the new matrix in the defects. Although both approaches helped cartilage regeneration, the results suggest that the specific composition of the rMSC-containing hydrogel permitted adequate bone regeneration, whereas the ELR-based hydrogel alone led to an excellent regeneration of hyaline cartilage. In conclusion, the ELR cross-linker solution can be easily delivered and forms a stable well-integrated hydrogel that supports infiltration and de novo matrix synthesis
PB Oxford University Press
SN 2056-3418
YR 2019
FD 2019
LK http://hdl.handle.net/10347/24487
UL http://hdl.handle.net/10347/24487
LA eng
NO Regenerative Biomaterials, Volume 6, Issue 6, December 2019, Pages 335–347, https://doi.org/10.1093/rb/rbz023
NO This project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No. 642687. The authors are grateful for the funding from the European Commission (NMP-2014-646075), the Spanish Government (PCIN-2015-010, MAT2015-68901-R, MAT2016-78903-R, MAT2016-79435-R), Junta de Castilla y León (VA015U16) and Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León. The authors also wish to thank Maria Victoria Saez Velasco for her important contribution to cell culture experiments
DS Minerva
RD 30 abr 2026