RT Journal Article
T1 A simple and sensitive approach to quantify methyl farnesoate in whole arthropods by matrix-solid phase dispersion and gas chromatography–mass spectrometry
A1 Montes Goyanes, Rosa
A1 Rodil Rodríguez, María del Rosario
A1 Neuparth, Teresa
A1 Quintana Álvarez, José Benito
A1 Cela Torrijos, Rafael
A1 Santos, Miguel M.
K1 Sample preparation
K1 Gas chromatography
K1 Matrix solid-phase dispersion
AB Methyl farnesoate (MF) is an arthropod hormone that plays a key role in the physiology of several arthropods’ classes being implicated in biological processes such as molting and reproduction. The development of an analytical technique to quantify the levels of this compound in biological tissues can be of major importance for the field of aquaculture/apiculture conservation and in endocrine disruption studies. Therefore, the aim of this study was to develop a simple and sensitive method to measure native levels of MF in the tissue of three representative species from different arthropods classes with environmental and/or economic importance. Thus, a new approach using whole organisms and the combination of matrix solid-phase dispersion with gas chromatography coupled to mass spectrometry was developed. This method allows quantifying endogenous MF at low levels (LOQs in the 1.2–3.1ng/g range) in three arthropod species, and could be expanded to additional arthropod classes. The found levels ranged between 2 and 12ng/g depending on the studied species and gender. The overall recovery of the method was evaluated and ranged between 69 and 96%.
PB Elsevier
YR 2017
FD 2017-06-02
LK http://hdl.handle.net/10347/31997
UL http://hdl.handle.net/10347/31997
LA eng
NO Rosa Montes, Rosario Rodil, Teresa Neuparth, Miguel M. Santos, Rafael Cela, José Benito Quintana, A simple and sensitive approach to quantify methyl farnesoate in whole arthropods by matrix-solid phase dispersion and gas chromatography–mass spectrometry, Journal of Chromatography A, Volume 1508, 2017, Pages 158-162
NO This work has been financially supported by the Ministry of Economy and Competitiveness, Spain(project no. CTM2014-56628-C3-2-R), the Galician Council of Culture, Education and Universities(ref. GRC2013-020) and FEDER/ERDF. T. Neuparth was supported by a Postdoctoral fellowshipfrom the Portuguese Science and Technology Foundation (FCT), ref. SFRH/BPD/77912/2011.
DS Minerva
RD 3 may 2026