RT Journal Article
T1 Characterization of the microbiota associated to Pecten maximus gonads using 454-pyrosequencing
A1 Lasa González, Aide
A1 Mira, Alex
A1 Camello-Castillo, Anny
A1 Belda-Ferre, Pedro
A1 López Romalde, Jesús
K1 Pecten maximus
K1 Aquaculture
K1 Gonads microbiota
K1 Molluscs pathogens
K1 Next-generation sequencing (NGS)
AB A next-generation sequencing (NGS) approach was used to study the microbiota associated to Pecten maximus broodstock, applying pyrosequencing of PCR-amplified V1-V4 16S rRNA gene regions. We analysed the resident bacterial communities in female and male scallop gonads before and after spawning. DNA samples were amplified and quality-filtered reads were assigned to family and genus taxonomic levels using the Ribosomal Database Project classifier. A total of 18,520 sequences were detected, belonging to 13 phyla, including Proteobacteria (55%), Bacteroidetes (11,7%), Firmicutes (3%), Actinobacteria (2%) and Spirochaetes (1,2%), and 110 genera. The major fraction of the sequences detected corresponded to Proteobacteria, Beta- and Gammaprotebacteria being the most abundant classes. The microbiota of P. maximus gonad harbour a wide diversity, however differences on male and female samples were observed, where female gonad samples show a larger number of genera and families. The dominant bacterial genera appeared to be Delftia, Acinetobacter, Hydrotalea, Aquabacterium, Bacillus, Sediminibacterium, Sphingomonas, and Pseudomonas that were present among the four analysed samples. This next generation sequencing technique, applied for the first time in P. maximus (great scallop) gonads was useful for the study of the bacterial communities in this mollusc, unravelling the great bacterial diversity in its microbiota. [Int Microbiol 19(2): 93-99(2016)].
PB Springer
YR 2016
FD 2016
LK https://hdl.handle.net/10347/46862
UL https://hdl.handle.net/10347/46862
LA eng
NO Lasa A, Mira A, Camelo-Castillo A, Belda-Ferre P, Romalde JL. Characterization of the microbiota associated to Pecten maximus gonads using 454-pyrosequencing. Int Microbiol. 2016 Jun;19(2):93-99. doi: 10.2436/20.1501.01.267. PMID: 27845496.
NO This work was supported in part by Project 245119 (REPROSEED) from KBBE-2009-1-2-11 Subprogram of the 7th Framework Programme (FP7), and grant AGL2013-42628-R from Ministerio de Economia y Competitividad (Spain). A.L. acknowledges the Ministerio de Ciencia e Innovación (Spain) for a research fellowship.
DS Minerva
RD 9 may 2026