RT Journal Article
T1 Functional Complexes of Angiotensin-Converting Enzyme 2 and Renin-Angiotensin System Receptors: Expression in Adult but Not Fetal Lung Tissue
A1 Franco, Rafael
A1 Lillo, Alejandro
A1 Rivas Santisteban, Rafael
A1 Rodríguez Pérez, Ana Isabel
A1 Reyes Resina, Irene
A1 Labandeira García, José Luis
A1 Navarro, Gemma
K1 COVID-19
K1 SARS-CoV-2 receptor
K1 RAS
K1 ACE2
K1 Angiotensin receptor
K1 Mas receptor
K1 Lung
AB Angiotensin-converting enzyme 2 (ACE2) is a membrane peptidase and a component of the renin-angiotensin system (RAS) that has been found in cells of all organs, including the lungs. While ACE2 has been identified as the receptor for severe acute respiratory syndrome (SARS) coronaviruses, the mechanism underlying cell entry remains unknown. Human immunodeficiency virus infects target cells via CXC chemokine receptor 4 (CXCR4)-mediated endocytosis. Furthermore, CXCR4 interacts with dipeptidyl peptidase-4 (CD26/DPPIV), an enzyme that cleaves CXCL12/SDF-1, which is the chemokine that activates this receptor. By analogy, we hypothesized that ACE2 might also be capable of interactions with RAS-associated G-protein coupled receptors. Using resonance energy transfer and cAMP and mitogen-activated protein kinase signaling assays, we found that human ACE2 interacts with RAS-related receptors, namely the angiotensin II type 1 receptor (AT1R), the angiotensin II type 2 receptor (AT2R), and the MAS1 oncogene receptor (MasR). Although these interactions led to various alterations of signal transduction, but, more importantly, ligand binding to AT1R resulted in the downregulation of ACE2 cell surface expression, while ligand binding to AT2R, but not to MasR, resulted in upregulation of ACE2 cell surface expression. Proximity ligation assays performed in situ revealed macromolecular complexes containing ACE2 and AT1R, AT2R or MasR in adult but not fetal mouse lung tissue. These findings highlight the relevance of RAS in SARS-CoV-2 infection and the role of ACE2-containing complexes as potential therapeutic targets
PB MDPI
YR 2020
FD 2020
LK http://hdl.handle.net/10347/24202
UL http://hdl.handle.net/10347/24202
LA eng
NO Franco, R.; Lillo, A.; Rivas-Santisteban, R.; Rodríguez-Pérez, A.I.; Reyes-Resina, I.; Labandeira-García, J.L.; Navarro, G. Functional Complexes of Angiotensin-Converting Enzyme 2 and Renin-Angiotensin System Receptors: Expression in Adult but Not Fetal Lung Tissue. Int. J. Mol. Sci. 2020, 21, 9602
NO The Molecular Neurobiology Laboratory of the University of Barcelona is considered a research group of excellence by the Regional Catalonian Government (grup consolidat #2017 SGR 1497)
DS Minerva
RD 30 abr 2026