RT Conference_Proceedings T1 Optimising butyrate and lactate yields fermenting xylose in a mixed culture system A1 Iglesias Riobó, Juan A1 Mauricio Iglesias, Miguel A1 Carballa Arcos, Marta K1 Butyrate K1 Lactate K1 Lignocellulosic biomass K1 Mixed culture K1 ph AB Despite being the second most abundant monomer in the earth, xylose fermentation has not been studied as thoroughly as glucose fermentation. This work studies the pH effect in xylose fermentation, focusing on lactic and butyric acid production as medium chain fatty acids (MCFA) precursors. Continuous reactor operation showed that changes within a slightly acidic pH range (pH 4.5 to pH 6) affect to VFA production but not their distribution, being butyric and acetic acid the main products regardless the pH value, while lactic acid was only detected when xylose or butyric acid concentrations dropped. In contrast, xylose conversion was negatively affected by low pH values. The use of lactic acid as substrate was assessed in continuous and batch reactors, being consumed and mainly converted into butyric acid. YR 2023 FD 2023-11-02 LK http://hdl.handle.net/10347/34747 UL http://hdl.handle.net/10347/34747 LA eng NO 5th IWA Resource Recovery Conference, 1-3 november 2023, Shenzhen, China NO This research was funded by Spanish Government through the CELL4CHEM project (PCI2021-121989, ERACoBioTech 3rd call). The authors belong to a Galician Competitive Research Group (ED431C-2021/37). DS Minerva RD 28 abr 2026