RT Journal Article
T1 Real-time investigation of dynamic protein crystallization in living cells
A1 Schonherr, R.
A1 Klinge, M.
A1 Rudolph, J. M.
A1 Fita, K.
A1 Rehders, D.
A1 Lubber, F.
A1 Schneegans, S.
A1 Majoul, I. V.
A1 Duszenko, M.
A1 Betzel, C.
A1 Brandariz Núñez, Alberto
A1 Martínez Costas, José Manuel
A1 Duden, R.
A1 Redecke, L.
AB X-ray crystallography requires sufficiently large crystals to obtain structural insights at atomic resolution, routinely obtained in vitro by time-consuming screening. Recently, successful data collection was reported from protein microcrystals grown within living cells using highly brilliant free-electron laser and third-generation synchrotron radiation. Here, we analyzed in vivo crystal growth of firefly luciferase and Green Fluorescent Protein-tagged reovirus μNS by live-cell imaging, showing that dimensions of living cells did not limit crystal size. The crystallization process is highly dynamic and occurs in different cellular compartments. In vivo protein crystallization offers exciting new possibilities for proteins that do not form crystals in vitro.
PB AIP Publishing
SN 2329-7778
YR 2015
FD 2015
LK http://hdl.handle.net/10347/22034
UL http://hdl.handle.net/10347/22034
LA eng
NO Schonherr, R., Klinge, M., Rudolph, J.M. et al. (2015). Real-time investigation of dynamic protein crystallization in living cells, "Structural Dynamics" 2, 041712
NO R.S. thanks the Graduate School for Computing in Medicine and Life Sciences at the University of Lübeck for funding. L.R., M.K., D.R., and C.B. thank the German Federal Ministry for Education and Research (BMBF) for funding (Grant Nos. 01KX0806 and 01KX0807). L.R., M.D., and C.B. acknowledge support from the BMBF in the context of the Röntgen-Angström-Cluster (Grant No. 05K12GU3). J.M.-C. and A.B.-N. acknowledge support from the Spanish Ministerio Economía y Competitividad (MINECO, Grant No. BFU2013-43513-R). I.V.M., R.D., and L.R. are grateful for support from the DFG Cluster of Excellence “Inflammation at Interfaces” (EXC 306)
DS Minerva
RD 26 abr 2026