RT Journal Article
T1 Immunohistochemical expression of E–cadherin in different tissues of the teleost fish Scophthalmus maximus
A1 Ronza, Paolo
A1 Villamarín Cid, José Antonio
A1 Méndez, Lucía
A1 Gómez Pardo, María Belén
A1 Bermúdez Pose, Roberto
A1 Quiroga Berdeal, María Isabel
K1 Immunohistochemistry
K1 Cadherin-1
K1 Cell junctions
K1 Epithelial barriers
K1 Mucosal health
AB E–cadherin is an evolutionary conserved protein, whose main role as the principal component of adherens junctions is supporting epithelial cell–cell adhesion. It is an essential molecule for the maintenance of the epithelial barrier function and the analysis of its immunohistochemical expression is a valuable resource in morphopathological, ontogenetic and pathogenesis studies in mammals. As well, there is an increasing understanding of the importance of E-cadherin in the physiology of the immune system and the development of the immune response. Mucosal health is a primary issue in aquaculture research; nevertheless, there is a lack of immunohistochemical studies of cell junction proteins in fish species. In this work, an immunohistochemical technique was optimized in Bouin- and formalin-fixed paraffin-embedded tissues of turbot Scophthalmus maximus, employing a commercial antibody raised against human E-cadherin. The specificity of the antibody in recognizing the molecule in this teleost species was tested by western blot and mass spectrometry-based proteomic analyses. The assays showed a good specificity and indicated that the antibody recognizes the well conserved cytoplasmic domain of the protein. Immunohistochemistry showed the localisation of E-cadherin at cell-cell contact in the epithelia of the different organs, between the hepatocytes and the pancreatic acinar cells, as well as in the reticulo-epithelial stroma of the thymus. Also, the immunoreaction was observed in the cells constituting the melano-macrophage centres in the spleen and kidney. No immunostaining was detected, as expected, only in the heart and brain. No significant difference was noticed between the two fixative used for collecting the tissues samples. This is the first description of E-cadherin immunohistochemical expression in several tissues of a teleost. The immunohistochemical technique represents a useful tool to be used in the different areas of fish health research
PB Elsevier
SN 0044-8486
YR 2019
FD 2019
LK http://hdl.handle.net/10347/26163
UL http://hdl.handle.net/10347/26163
LA eng
NO Aquaculture, 501 (2019), 465-472
NO This work was supported by the Spanish Ministry of Economy, Industry and Competitiveness, and the European Regional Development Fund (ERDF) through the projects AGL2015–67039–C3–1–R and AGL2015–67039–C3–3–R
DS Minerva
RD 4 may 2026