RT Journal Article
T1 Natural and cross-inducible anti-SIV antibodies in Mauritian cynomolgus macaques
A1 Li, Hongzhao
A1 Nykoluk, Mikaela
A1 Li, Lin
A1 Liu, Lewis R.
A1 Omange, Robert W.
A1 Soule, Geoff
A1 Schroeder, Lukas T.
A1 Toledo, Nikki
A1 Kashem, Mohammad Abul
A1 Correia Pinto, Jorge F.
A1 Liang, Binhua
A1 Schultz-Darken, Nancy
A1 Alonso Fernández, María José
A1 Whitney, James B.
A1 Plummer, Francis A.
A1 Luo, Ma
AB Cynomolgus macaques are an increasingly important nonhuman primate model for HIV vaccine research. SIV-free animals without pre-existing anti-SIV immune responses are generally needed to evaluate the effect of vaccine-induced immune responses against the vaccine epitopes. Here, in order to select such animals for vaccine studies, we screened 108 naïve female Mauritian cynomolgus macaques for natural (baseline) antibodies to SIV antigens using a Bio-Plex multiplex system. The antigens included twelve 20mer peptides overlapping the twelve SIV protease cleavage sites (-10/+10), respectively (PCS peptides), and three non-PCS Gag or Env peptides. Natural antibodies to SIV antigens were detected in subsets of monkeys. The antibody reactivity to SIV was further confirmed by Western blot using purified recombinant SIV Gag and Env proteins. As expected, the immunization of monkeys with PCS antigens elicited anti-PCS antibodies. However, unexpectedly, antibodies to non-PCS peptides were also induced, as shown by both Bio-Plex and Western blot analyses, while the non-PCS peptides do not share sequence homology with PCS peptides. The presence of natural and vaccine cross-inducible SIV antibodies in Mauritian cynomolgus macaques should be considered in animal selection, experimental design and result interpretation, for their best use in HIV vaccine research
PB PLOS
YR 2017
FD 2017
LK http://hdl.handle.net/10347/21611
UL http://hdl.handle.net/10347/21611
LA eng
NO Li H, Nykoluk M, Li L, Liu LR, Omange RW, Soule G, et al. (2017) Natural and cross-inducible anti-SIV antibodies in Mauritian cynomolgus macaques. PLoS ONE 12(10): e0186079. https://doi.org/10.1371/journal.pone.0186079
NO This work was supported by an NIH grant (R01AI111805), a CIHR/CHVI bridging grant and funding from National Microbiology Laboratory of Canada
DS Minerva
RD 30 abr 2026