RT Dissertation/Thesis
T1 Analysis of the processing of complex recombination intermediates by structure-selective endonucleases
A1 Carreira Rodríguez, Raquel
K1 Genome stability
K1 DNA repair
K1 Homologous recombination
K1 Structure-selective endonucleases
AB DNA repair by homologous recombination involves the formation ofbranched intermediates that can constitute a source of genome instability when untimely processed. To preventthese structures from interfering with chromosome segregation, cells rely on the action of the structure-selectiveendonucleases Mus81-Mms4 and Yen1. Here, we employed a biochemical approach to show that bothendonucleases can process the central intermediate of this pathway, the displacement loop (D-loop). Usingsynthetic and enzymatically reconstituted D-loops, we mapped their incision sites and recapitulated for the first timethe formation of a half-crossover precursor in vitro. This implies that the concurrent action of Mus81-Mms4 andYen1 on a D-loop may lead to the generation of complex chromosomal rearrangements.
YR 2023
FD 2023
LK http://hdl.handle.net/10347/30418
UL http://hdl.handle.net/10347/30418
LA eng
DS Minerva
RD 24 abr 2026