RT Journal Article
T1 QTL detection for Aeromonas salmonicida resistance related traits in turbot (Scophthalmus maximus)
A1 Rodríguez Ramilo, Silvia Teresa
A1 Toro Ibáñez, Miguel Ángel
A1 Bouza Fernández, María Carmen
A1 Hermida Prieto, Miguel
A1 Gómez Pardo, María Belén
A1 Cabaleiro, Santiago
A1 Martínez Portela, Paulino
A1 Fernández Martín, Jesús
K1 Quantitative Trait Locus
K1 Atlantic Salmon
K1 Differentially Express
K1 Differentially Express Gene
K1 Quantitative Trait Locus Detection
AB BackgroundInteractions between fish and pathogens, that may be harmless under natural conditions, often result in serious diseases in aquaculture systems. This is especially important due to the fact that the strains used in aquaculture are derived from wild strains that may not have had enough time to adapt to new disease pressures. The turbot is one of the most promising European aquaculture species. Furunculosis, caused by the bacterium Aeromonas salmonicida, produces important losses to turbot industry. An appealing solution is to achieve more robust broodstock, which can prevent or diminish the devastating effects of epizooties. Genomics strategies have been developed in turbot to look for candidate genes for resistance to furunculosis and a genetic map with appropriate density to screen for genomic associations has been also constructed. In the present study, a genome scan for QTL affecting resistance and survival to A. salmonicida in four turbot families was carried out. The objectives were to identify consistent QTL using different statistical approaches (linear regression and maximum likelihood) and to locate the tightest associated markers for their application in genetic breeding strategies.ResultsSignificant QTL for resistance were identified by the linear regression method in three linkage groups (LGs 4, 6 and 9) and for survival in two LGs (6 and 9). The maximum likelihood methodology identified QTL in three LGs (5, 6 and 9) for both traits. Significant association between disease traits and genotypes was detected for several markers, some of them explaining up to 17% of the phenotypic variance. We also identified candidate genes located in the detected QTL using data from previously mapped markers.ConclusionsSeveral regions controlling resistance to A. salmonicida in turbot have been detected. The observed concordance between different statistical methods at particular linkage groups gives consistency to our results. The detected associated markers could be useful for genetic breeding strategies. A finer mapping will be necessary at the detected QTL intervals to narrow associations and around the closely associated markers to look for candidate genes through comparative genomics or positional cloning strategies. The identification of associated variants at specific genes will be essential, together with the QTL associations detected in this study, for future marker assisted selection programs.
PB BMC
SN 1471-2164
YR 2011
FD 2011
LK http://hdl.handle.net/10347/22119
UL http://hdl.handle.net/10347/22119
LA eng
NO Rodríguez-Ramilo, S.T., Toro, M.A., Bouza, C. et al. QTL detection for Aeromonas salmonicida resistance related traits in turbot (Scophthalmus maximus). BMC Genomics 12, 541 (2011). https://doi.org/10.1186/1471-2164-12-541
NO This study was supported by the Spanish government projects (Consolider Ingenio Aquagenomics: CSD200700002; MCI: CGL2009-13278-C02 and AGL2009-13273), and Xunta de Galicia and Fondos Feder (Grupos de Referencia Competitiva, 2010/80). B. G. P. was supported by an Isidro Parga Pondal research fellowship from Xunta de Galicia (Spain)
DS Minerva
RD 28 abr 2026