RT Journal Article
T1 Removal of Estrogenic Compounds from Filtered Secondary Wastewater Effluent in a Continuous Enzymatic Membrane Reactor. Identification of Biotransformation Products
A1 Lloret, Lucía
A1 Eibes González, Gemma María
A1 Moreira Vilar, María Teresa
A1 Feijoo Costa, Gumersindo
A1 Lema Rodicio, Juan Manuel
AB In the present study, a novel and efficient technology based on the use of an oxidative enzyme was developed to perform the continuous removal of estrogenic compounds from polluted wastewaters. A 2 L enzymatic membrane reactor (EMR) was successfully operated for 100 h with minimal requirements of laccase for the transformation of estrone (E1), 17β-estradiol (E2), and 17α-ethinylestradiol (EE2)from both buffer solution and real wastewater (filtered secondary effluent). When the experiments were performed at high and low concentrations of the target compounds, 4 mg/L and 100 μg/L, not only high removal yields (80–100%) but also outstanding reduction of estrogenicity (about 84–95%) were attained. When the EMR was applied for the treatment of municipal wastewaters with real environmental concentrations of the different compounds (0.29–1.52 ng/L), excellent results were also achieved indicating the high efficiency and potential of the enzymatic reactor system. A second goal of this study relied on the identification of the transformation products to elucidate the catalytic mechanism of estrogens’ transformation by laccase. The formation of dimers and trimers of E1, E2, and EE2, as well as the decomposition of E2 into E1 by laccase-catalyzed treatment, has been demonstrated by liquid chromatography atmospheric pressure chemical ionization (LC-APCI) analysis and confirmed by determination of accurate masses through liquid chromatography electrospray time-of-flight mass spectrometry (LC-ESI-TOF). Dimeric products of E2 and EE2 were found even when operating at environmental concentrations. Moreover, the reaction pathways of laccase-catalyzed transformation of E2 were proposed.
PB ACS Publications
YR 2013
FD 2013-04-01
LK https://hdl.handle.net/10347/39261
UL https://hdl.handle.net/10347/39261
LA eng
NO Lloret, L. et al. Removal of Estrogenic Compounds from Filtered SecondaryWastewater Effluent in a Continuous Enzymatic Membrane Reactor.Identification of Biotransformation Products, Environ. Sci. Technol. 2013, 47, 9, 4536–4543
DS Minerva
RD 28 abr 2026