RT Journal Article
T1 A modification of the resazurin cell viability assay, suitable for the quantification of lactic acid producing bacteria
A1 Gómez Calvo, Lorena
A1 Villarino Fernández, Rosa Antía
A1 Rama, José Luis R.
A1 Abril, Ana G.
A1 Miguel Bouzas, María Trinidad de
K1 Cytisus scoparius
K1 Fluorescence
K1 Lactic acid bacteria
K1 Prebiotics
K1 Resazurin
AB The conventional resazurin cell viability assay is based on the conversion of resazurin into the fluorescent molecule resorufin in the presence of metabolically active cells. Nevertheless, this method encounters limitations derived from medium acidification by lactic acid bacteria. Here we propose a two-step modification of the resazurin method for accurate lactic acid bacteria growth and prebiotic assessment without pH interference. Using a polyphenolic extract from Cytisus scoparius as a test prebiotic, this study demonstrates the efficacy of a two-step resazurin assay in quantifying lactic acid bacteria growth underscoring the importance of innovative methodologies for prebiotics screening.
PB Elsevier
YR 2024
FD 2024-12-20
LK https://hdl.handle.net/10347/46394
UL https://hdl.handle.net/10347/46394
LA eng
NO Calvo, L. G., Villarino, R.-A., Rama, J. L. R., Abril, A. G., & de Miguel, T. (2025). A modification of the resazurin cell viability assay, suitable for the quantification of lactic acid producing bacteria. LWT, 215. https://doi.org/10.1016/J.LWT.2024.117259
DS Minerva
RD 19 abr 2026