RT Journal Article
T1 In situ functionalized polymers for siRNA delivery
A1 Martínez Costas, José Manuel
A1 Fernández Trillo, Francisco
A1 Montenegro García, Javier
A1 Granja Guillán, Juan Ramón
A1 Priegue Caamaño, Juan Manuel
A1 Crisan, Daniel
K1 Lipid bilayer membranes
K1 Polymers
K1 siRNA delivery
K1 Supramolecular chemistry
K1 Vesicles
AB A new method is reported herein for screening the biological activity of functional polymers across a consistent degree of polymerization and in situ, that is, under aqueous conditions and without purification/isolation of candidate polymers. In brief, the chemical functionality of a poly(acryloyl hydrazide) scaffold was activated under aqueous conditions using readily available aldehydes to obtain amphiphilic polymers. The transport activity of the resulting polymers can be evaluated in situ using model membranes and living cells without the need for tedious isolation and purification steps. This technology allowed the rapid identification of a supramolecular polymeric vector with excellent efficiency and reproducibility for the delivery of siRNA into human cells (HeLa-EGFP). The reported method constitutes a blueprint for the high-throughput screening and future discovery of new polymeric functional materials with important biological applications
PB WILEY-VCH Verlag GmbH
SN 1433-7851
YR 2016
FD 2016-04-21
LK http://hdl.handle.net/10347/14912
UL http://hdl.handle.net/10347/14912
LA eng
NO J. M. Priegue, D. N. Crisan, J. Martínez-Costas, J. R. Granja, F. Fernandez-Trillo, J. Montenegro, Angew. Chem. Int. Ed. 2016, 55, 7492
NO NOTICE:This is the peer-reviewed version of the following article:Juan M. Priegue, Daniel N. Crisan, José Martínez-Costas, Juan R. Granja, Francisco Fernandez-Trillo, and Javier Montenegro (2016),“In situ Functionalized Polymers for siRNA Delivery ; Angew. Chem. Int. Ed., 55, 7492–7495 [doi: 10.1002/anie.201601441]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for self-archiving
NO Royal Society. Grant Number: IE130688Spanish Ministry of Economy and Competitiveness. Grant Numbers: CTQ2014-59646-R, CTQ2013-43264-R, BFU2013-43513-RBirmingham Science CityEuropean Regional Development FundRoyal Society. Grant Number: RG140273University of BirminghamMECMINECOERC. Grant Number: DYNAP-677786
DS Minerva
RD 24 abr 2026