RT Dissertation/Thesis
T1 Fast, Affordable and multiplexed foodborne pathogen detection on miniaturized devices
A1 Azinheiro, Sarah Gaspar Ferreira
K1 Foodborne pathogens
K1 Food analysis
K1 Food safety
K1 Improved methodology
K1 sample pre-treatment
K1 DNA amplification
K1 Isothermal amplification
AB Food poisoning is a global public health concern affecting, not onlydeveloping, but also developed countries, where several pathogens are responsible for these foodborne diseasescausing high number of hospitalization and death. The objective of this thesis was to develop an improvedmethodology for the multiplex detection of Salmonella spp., Shiga Toxin-producing E. coli (STEC) and L.monocytogenes, based in DNA detection, and its integration in a miniaturized device. To achieve this goal, thedifferent steps of the analysis, including the sample pre-treatment, DNA amplification and visualization of theresults were addressed in order to reduce the time of analysis, reduce the cost, and allow naked-eye detection. Themethodology developed in this project allowed for the multiplex detection of the three targets, reducing the time ofanalysis to only 9 h vs 7 days by traditional approaches with similar sensitivity and specificity to qPCR.Furthermore, the possibility to integrate the amplification step in a miniaturize device opens the door for thedevelopment of Point-of-Care systems.
YR 2022
FD 2022
LK http://hdl.handle.net/10347/29339
UL http://hdl.handle.net/10347/29339
LA eng
DS Minerva
RD 28 abr 2026