RT Journal Article
T1 Gold half-shell-coated paclitaxel-loaded PLGA nanoparticles for the targeted chemo-photothermal treatment of cancer
A1 Ibarra, Jaime
A1 Encinas Basurto, David
A1 Almada, Mario
A1 Juárez, Josué
A1 Valdez, Miguel Ángel
A1 Barbosa Fernández, Silvia
A1 Taboada Antelo, Pablo
K1 Paclitaxel
K1 PLGA
K1 Half shell
K1 CyRGDk peptide
K1 Chemo-photothermal therapy
AB Conventional cancer therapies suffer from nonspecificity, drug resistance, and a poor bioavailability, which trigger severe side effects. To overcome these disadvantages, in this study, we designed and evaluated the in vitro potential of paclitaxel-loaded, PLGA-gold, half-shell nanoparticles (PTX-PLGA/Au-HS NPs) conjugated with cyclo(Arg-Gly-Asp-Phe-Lys) (cyRGDfk) as a targeted chemo-photothermal therapy system in HeLa and MDA-MB-231 cancer cells. A TEM analysis confirmed the successful gold half-shell structure formation. High-performance liquid chromatography showed an encapsulation efficiency of the paclitaxel inside nanoparticles of more than 90%. In the release study, an initial burst release of about 20% in the first 24 h was observed, followed by a sustained drug release for a period as long as 10 days, reaching values of about 92% and 49% for NPs with and without near infrared laser irradiation. In in vitro cell internalization studies, targeted nanoparticles showed a higher accumulation than nontargeted nanoparticles, possibly through a specific interaction of the cyRGDfk with their homologous receptors, the ανβ3 y ανβ5 integrins on the cell surface. Compared with chemotherapy or photothermal treatment alone, the combined treatment demonstrated a synergistic effect, reducing the cell viability to 23% for the HeLa cells and 31% for the MDA-MB-231 cells. Thus, our results indicate that these multifuncional nanoparticles can be considered to be a promising targeted chemo-photothermal therapy system against cancer.
PB MDPI
YR 2023
FD 2023-07-08
LK https://hdl.handle.net/10347/43939
UL https://hdl.handle.net/10347/43939
LA eng
NO Ibarra, J.; Encinas-Basurto, D.; Almada, M.; Juárez, J.; Valdez, M.A.; Barbosa, S.; Taboada, P. Gold Half-Shell-Coated Paclitaxel-Loaded PLGA Nanoparticles for the Targeted Chemo-Photothermal Treatment of Cancer. Micromachines 2023, 14, 1390. https://doi.org/10.3390/mi14071390
DS Minerva
RD 24 abr 2026