RT Journal Article
T1 Ultrasonication followed by enzymatic hydrolysis as a sample pre-treatment for the determination of Ag nanoparticles in edible seaweed by SP-ICP-MS
A1 López Mayán, Juan José
A1 Álvarez Fernández, Blanca
A1 Peña Vázquez, Elena María
A1 Barciela Alonso, María Carmen
A1 Moreda Piñeiro, Antonio
A1 Bermejo Barrera, Pilar
K1 Seaweed
K1 Silver nanoparticles
K1 Ultrasonication
K1 Enzymatic hydrolysis
K1 Single-particle-ICP-MS
AB Seaweed can bioaccumulate nanomaterials that would be transferred to the trophic chain. This work describes the optimization of a method for the separation of silver nanoparticles (AgNPs) from seaweed using an ultrasound-assisted enzymatic hydrolysis method and ulterior determination by single particle inductively coupled plasma mass spectrometry (SP-ICP-MS). The following parameters affecting the isolation of AgNPs were optimized using a Palmaria palmata (red seaweed) sample previously exposed to AgNPs: type of sonication (bath vs. ultrasonic probe), ultrasound amplitude, sonication time, sonication mode (pulsed vs. continuous sonication), concentration of the enzymes mixture (Macerozyme R-10®), and enzymatic hydrolysis time. The stability of AgNPs during extraction was tested by transmission electron microscopy (TEM) and using a standard of 15 nm of polyvinylpyrrolidone (PVP)-coated AgNPs analyzed by SP-ICP-MS. The analytical performance was evaluated with good results. For total Ag determination, the limits of detection and quantification were 2.2 and 7.7 ng g−1, respectively; and for AgNPs determination, the limits of detection in size and number were 14 nm and 4.34 × 107 part g−1, respectively. Besides, the matrix effect, the repeatability and the analytical recovery were also studied. Finally, the method was applied to the analysis of several red (Palmaria palmata) and green (Ulva sp.) seaweed samples
PB Elsevier
YR 2022
FD 2022
LK http://hdl.handle.net/10347/29161
UL http://hdl.handle.net/10347/29161
LA eng
NO Talanta  247 (2022) 123556
NO The authors wish to acknowledge the financial support of Ministerio de Economía y Competitividad (project INNOVANANO, reference RT2018-099222-B-100), European Union (INTERREG Atlantic Area, project NANOCULTURE, reference EAPA590/2018), and Xunta de Galicia (Grupo de Referencia Competitiva, grant number ED431C2018/19)
DS Minerva
RD 24 abr 2026