RT Journal Article
T1 Loss of Active Neurogenesis in the Adult Shark Retina
A1 Hernández Núñez, Ismael
A1 Robledo Sánchez, Diego
A1 Mayeur, Hélène
A1 Mazan, Sylvie
A1 Sánchez Piñón, Laura
A1 Adrio Fondevila, Fátima
A1 Barreiro Iglesias, Antón
A1 Candal Suárez, Eva María
K1 Retina
K1 Neurogenesis
K1 Cell proliferation
K1 RNA-seq
K1 Mitosis
K1 Progenitor cells
K1 Cartilaginous fish
AB Neurogenesis is the process by which progenitor cells generate new neurons. As development progresses neurogenesis becomes restricted to discrete neurogenic niches, where it persists during postnatal life. The retina of teleost fishes is thought to proliferate and produce new cells throughout life. Whether this capacity may be an ancestral characteristic of gnathostome vertebrates is completely unknown. Cartilaginous fishes occupy a key phylogenetic position to infer ancestral states fixed prior to the gnathostome radiation. Previous work from our group revealed that the juvenile retina of the catshark Scyliorhinus canicula, a cartilaginous fish, shows active proliferation and neurogenesis. Here, we compared the morphology and proliferative status of the retina in catshark juveniles and adults. Histological and immunohistochemical analyses revealed an important reduction in the size of the peripheral retina (where progenitor cells are mainly located), a decrease in the thickness of the inner nuclear layer (INL), an increase in the thickness of the inner plexiform layer and a decrease in the cell density in the INL and in the ganglion cell layer in adults. Contrary to what has been reported in teleost fish, mitotic activity in the catshark retina was virtually absent after sexual maturation. Based on these results, we carried out RNA-Sequencing (RNA-Seq) analyses comparing the retinal transcriptome of juveniles and adults, which revealed a statistically significant decrease in the expression of many genes involved in cell proliferation and neurogenesis in adult catsharks. Our RNA-Seq data provides an excellent resource to identify new signaling pathways controlling neurogenesis in the vertebrate retina
PB Frontiers
YR 2021
FD 2021
LK http://hdl.handle.net/10347/24697
UL http://hdl.handle.net/10347/24697
LA eng
NO Hernández-Núñez I, Robledo D, Mayeur H, Mazan S, Sánchez L, Adrio F, Barreiro-Iglesias A and Candal E (2021) Loss of Active Neurogenesis in the Adult Shark Retina. Front. Cell Dev. Biol. 9:628721. doi: 10.3389/fcell.2021.628721
NO Funded by the Ministerio de Economía Industria y Competitividad (to EC; grant number BFU-2017-89861-P) and Xunta de Galicia Predoctoral Fellowship (to IH-N; grant number ED 481 A 2018 216). Both grants were partially financed by the European Social Fund
DS Minerva
RD 29 abr 2026