RT Journal Article
T1 Biofilm formation as a novel phenotypic feature of adherent-invasive Escherichia coli (AIEC)
A1 Martínez Medina, Margarita
A1 Naves, Plínio
A1 Blanco Álvarez, Jorge
A1 Aldeguer, Xavier
A1 Blanco Álvarez, Jesús Eulogio
A1 Blanco Álvarez, Miguel
A1 Ponte, Carmen
A1 Soriano, Francisco
A1 Darfeuille Michaud, Arlette
A1 García Gil, L. Jesús
K1 Promote Tumour Necrosis Factor
K1 Motile Strain
K1 AIEC Strain
K1 ATCC Accession Number
K1 Gene ibeA
AB BackgroundCrohn's disease (CD) is a high morbidity chronic inflammatory disorder of unknown aetiology. Adherent-invasive Escherichia coli (AIEC) has been recently implicated in the origin and perpetuation of CD. Because bacterial biofilms in the gut mucosa are suspected to play a role in CD and biofilm formation is a feature of certain pathogenic E. coli strains, we compared the biofilm formation capacity of 27 AIEC and 38 non-AIEC strains isolated from the intestinal mucosa. Biofilm formation capacity was then contrasted with the AIEC phenotype, the serotype, the phylotype, and the presence of virulence genes.ResultsSpecific biofilm formation (SBF) indices were higher amongst AIEC than non-AIEC strains (P = 0.012). In addition, 65.4% of moderate to strong biofilms producers were AIEC, whereas 74.4% of weak biofilm producers were non-AIEC (P = 0.002). These data indicate that AIEC strains were more efficient biofilm producers than non-AIEC strains. Moreover, adhesion (P = 0.009) and invasion (P = 0.003) indices correlated positively with higher SBF indices. Additionally, motility (100%, P < 0.001), H1 type flagellin (53.8%, P < 0.001), serogroups O83 (19.2%, P = 0.008) and O22 (26.9%, P = 0.001), the presence of virulence genes such as sfa/focDE (38.5%, P = 0.003) and ibeA (26.9%, P = 0.017), and B2 phylotype (80.8%, P < 0.001) were frequent characteristics amongst biofilm producers.ConclusionThe principal contribution of the present work is the finding that biofilm formation capacity is a novel, complementary pathogenic feature of the recently described AIEC pathovar. Characterization of AIEC specific genetic determinants, and the regulatory pathways, involved in biofilm formation will likely bring new insights into AIEC pathogenesis.
PB BMC
SN 1471-2180
YR 2009
FD 2009
LK http://hdl.handle.net/10347/22743
UL http://hdl.handle.net/10347/22743
LA eng
NO Martinez-Medina, M., Naves, P., Blanco, J. et al. Biofilm formation as a novel phenotypic feature of adherent-invasive Escherichia coli(AIEC). BMC Microbiol 9, 202 (2009). https://doi.org/10.1186/1471-2180-9-202
NO This work was partially supported by the Spanish Ministry of Education and Science (SAF2006-00414), the Spanish Ministry of Health and Consumer Affairs (REIPI RD06/0008-1018 and FIS PI060059), the Autonomous Government of Galicia (Xunta de Galicia, 2007/000044-0, PGIDIT065TAL26101PR, 07MRU036261PR), and the European Union (Program Alban, E05D055472BR)
DS Minerva
RD 4 may 2026