RT Journal Article
T1 Vesicles Shed by Pathological Murine Adipocytes Spread Pathology: Characterization and Functional Role of Insulin Resistant/Hypertrophied Adiposomes
A1 Camino, Tamara
A1 Lago Baameiro, Nerea
A1 Bravo López, Susana Belén
A1 Sueiro, Aurelio M.
A1 Couto, Iván
A1 Santos, Fernando
A1 Baltar, Javier
A1 Casanueva Freijo, Felipe
A1 Pardo, María
K1 Extracellular vesicles
K1 Adipocytes
K1 Insulin resistance
K1 Lipid hypertrophy
K1 Inflammation
K1 Proteomics
K1 SWATH
AB Extracellular vesicles (EVs) have recently emerged as a relevant way of cell to cell communication, and its analysis has become an indirect approach to assess the cell/tissue of origin status. However, the knowledge about their nature and role on metabolic diseases is still very scarce. We have established an insulin resistant (IR) and two lipid (palmitic/oleic) hypertrophied adipocyte cell models to isolate EVs to perform a protein cargo qualitative and quantitative Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH) analysis by mass spectrometry. Our results show a high proportion of obesity and IR-related proteins in pathological EVs; thus, we propose a panel of potential obese adipose tissue EV-biomarkers. Among those, lipid hypertrophied vesicles are characterized by ceruloplasmin, mimecan, and perilipin 1 adipokines, and those from the IR by the striking presence of the adiposity and IR related transforming growth factor-beta-induced protein ig-h3 (TFGBI). Interestingly, functional assays show that IR and hypertrophied adipocytes induce differentiation/hypertrophy and IR in healthy adipocytes through secreted EVs. Finally, we demonstrate that lipid atrophied adipocytes shed EVs promote macrophage inflammation by stimulating IL-6 and TNFα expression. Thus, we conclude that pathological adipocytes release vesicles containing representative protein cargo of the cell of origin that are able to induce metabolic alterations on healthy cells probably exacerbating the disease once established
PB MDPI
YR 2020
FD 2020
LK http://hdl.handle.net/10347/23848
UL http://hdl.handle.net/10347/23848
LA eng
NO Camino, T.; Lago-Baameiro, N.; Bravo, S.B.; Sueiro, A.; Couto, I.; Santos, F.; Baltar, J.; Casanueva, F.F.; Pardo, M. Vesicles Shed by Pathological Murine Adipocytes Spread Pathology: Characterization and Functional Role of Insulin Resistant/Hypertrophied Adiposomes. Int. J. Mol. Sci. 2020, 21, 2252
NO This research was funded by Instituto de Salud Carlos III-FEDER, grant number PI16/01212
DS Minerva
RD 24 abr 2026