RT Journal Article
T1 Optimization of extraction conditions and fatty acid characterization of Lactobacillus pentosus cell-bound biosurfactant/bioemulsifier
A1 Vecino, Xanel
A1 Barbosa Pereira, Letricia
A1 Devesa Rey, Rosa
A1 Cruz Freire, José Manuel
A1 Moldes, Ana B.
K1 Lactobacillus pentosus
K1 Biosurfactant
K1 Fatty acids
K1 Rosemary emulsion
AB BACKGROUNDThere is currently much interest in the use of natural biosurfactants and bioemulsifiers, mainly in the cosmetic, pharmaceutical and food industries. However, there are no studies on the optimization of the extraction conditions of cell-bound biosurfactants. In this work, a biosurfactant with emulsifier properties was extracted from Lactobacillus pentosus cells, under different extraction conditions, and characterized.RESULTSDuring extraction, the most influential independent variable, concerning the emulsifying capacity of biosurfactant, was the operation time, followed by temperature and salt concentration. Biosurfactant from L. pentosus was evaluated by Fourier transform infrared spectroscopy and the composition of fatty acids was analyzed by gas chromatography–mass spectrometry. The hydrophobic chain of the biosurfactant from L. pentosus comprises 548 g kg−1 linoelaidic acid (C18:2), 221 g kg−1 oleic or elaidic acid (C18:1), 136 g kg−1 palmitic acid (C16) and 95 g kg−1 stearic acid (C18). In addition, emulsions of water and rosemary oil were stabilized with a biosurfactant produced by L. pentosus and compared with emulsions stabilized with polysorbate 20.CONCLUSIONThe optimum extraction conditions of biosurfactant were achieved at 45 °C at 120 min and using 9 g kg−1 of salt. In all the assays biosurfactant from L. pentosus yielded more stable emulsions and higher emulsion volumes than polysorbate 20. © 2014 Society of Chemical Industry
PB Wiley
SN 0022-5142
YR 2015
FD 2015
LK https://hdl.handle.net/10347/39408
UL https://hdl.handle.net/10347/39408
LA eng
NO Vecino, X., Barbosa-Pereira, L., Devesa-Rey, R., Cruz, J. M., & Moldes, A. B. (2015). Optimization of extraction conditions and fatty acid characterization of Lactobacillus pentosus cell-bound biosurfactant/bioemulsifier. Journal of the Science of Food and Agriculture, 95(2), 313–320. https://doi.org/10.1002/jsfa.6720
NO Xunta de Galicia (project GPC, ref.CN2012/277)
NO España. Ministerio de Economía y Competitividad (project CTM2012-31873)
DS Minerva
RD 28 abr 2026