RT Journal Article
T1 Acute Toxicity Assessment: Macroscopic and Ultrastructural Effects in Mice Treated with Oral Tetrodotoxin
A1 Abal Camaño, Paula
A1 Louzao Ojeda, María del Carmen
A1 Vilariño del Río, Natalia
A1 Rodríguez Vieytes, Mercedes
A1 Botana López, Luis Miguel
K1 Tetrodotoxin
K1 Macroscopic alterations
K1 Ultrastructural changes
K1 Gastrointestinal effect
K1 Mitochondria swelling
K1 Rough endoplasmic reticulum swelling
AB Tetrodotoxin (TTX) is an extremely toxic marine compound produced by different genera of bacteria that can reach humans through ingestion mainly of pufferfish but also of other contaminated fish species, marine gastropods or bivalves. TTX blocks voltage-gated sodium channels inhibiting neurotransmission, which in severe cases triggers cardiorespiratory failure. Although TTX has been responsible for many human intoxications limited toxicological data are available. The recent expansion of TTX from Asian to European waters and diversification of TTX-bearing organisms entail an emerging risk of food poisoning. This study is focused on the acute toxicity assessment of TTX administered to mice by oral gavage following macroscopic and microscopic studies. Necropsy revealed that TTX induced stomach swelling 2 h after administration, even though no ultrastructural alterations were further detected. However, transmission electron microscopy images showed an increase of lipid droplets in hepatocytes, swollen mitochondria in spleens, and alterations of rough endoplasmic reticulum in intestines as hallmarks of the cellular damage. These findings suggested that gastrointestinal effects should be considered when evaluating human TTX poisoning.
PB MDPI
YR 2019
FD 2019
LK http://hdl.handle.net/10347/21203
UL http://hdl.handle.net/10347/21203
LA eng
NO Abal, P., Louzao, M.C., Vilariño, N., Vyeites, M.R., Botana, L.M. (2019). Acute Toxicity Assessment: Macroscopic and Ultrastructural Effects in Mice Treated with Oral Tetrodotoxin. "Toxins", 11(6), 305
NO The research leading to these results has received funding from the following FEDER cofunded-grants. From CDTI and Technological Funds, supported by Ministerio de Economía, Industria y Competitividad, AGL2016-78728-R (AEI/FEDER, UE) AGL2014-58210-R, ISCIII/PI16/01830 and RTC-2016-5507-2, ITC-20161072. From Conselleria de Cultura, Educacion e Ordenación Universitaria, Xunta de Galicia, 2017 GRC GI-1682 (ED431C 2017/01). From European Union POCTEP 0161-Nanoeaters -1-E-1, Interreg AlertoxNet EAPA-317-2016, Interreg Agritox EAPA-998-2018, and H2020 778069-EMERTOX
DS Minerva
RD 30 abr 2026