RT Journal Article
T1 Cytotoxin- and chemotaxis-genes cooperate to promote adhesion of photobacterium damselae subsp. damselae
A1 Von Hoven, Gisela
A1 Neukirch, Claudia
A1 Meyenburg, Martina
A1 Schmidt, Sabine
A1 Vences Lorenzo, Ana
A1 Rodríguez Osorio, Carlos
A1 Husmann, Matthias
A1 Rivas Fontenla, Amable José
K1 Photobacterium damselae subsp. damselae
K1 Pore forming toxin
K1 Phospholipase D
K1 Chemotaxis
K1 CheA
K1 Swimming
K1 Salinity
K1 Adhesion
AB Photobacterium damselae subsp. damselae (Pdd) is an emerging pathogen of marine animals that sometimes causes serious infections in humans. Two related pore forming toxins, phobalysins P and C, and damselysin, a phospholipase D, confer strong virulence of Pdd in mice. Because infections by Pdd are typically caused following exposure of wounds to sea water we investigated how salinity impacts toxin activity, swimming, and association of Pdd with epithelial cells. These activities were low when bacteria were pre-cultured in media with 3.5% NaCl, the global average salinity of sea water. In contrast, lower salinity increased swimming of wild type Pdd peaking at 2% NaCl, hemolysis, and association with epithelial cells peaking at 1–1.5%. Previously, we have found that hemolysin genes enhance the association of Pdd with epithelial cells, but the underlying mechanisms have remained ill-defined. We here searched for potential links between hemolysin-production, chemotaxis and association of Pdd with target cells at varying salt concentrations. Unexpectedly, disruption of chemotaxis regulator cheA not only affected bacterial swimming and association with epithelial cells at intermediate to low salinity, but also reduced the production of plasmid-encoded phobalysin (PhlyP). The results thus reveal unforeseen links between chemotaxis regulators, a pore forming toxin and the association of a marine bacterium with target cells
PB Frontiers Media
YR 2018
FD 2018
LK http://hdl.handle.net/10347/22628
UL http://hdl.handle.net/10347/22628
LA eng
NO von Hoven G, Neukirch C, Meyenburg M, Schmidt S, Vences A, Osorio CR, Husmann M and Rivas AJ (2018) Cytotoxinand Chemotaxis-Genes Cooperate to Promote Adhesion of Photobacterium damselae subsp. damselae. Front. Microbiol. 9:2996. doi: 10.3389/fmicb.2018.02996
NO The work was supported by an intramural grant of the University Medical Center Mainz to AR. Work in the CO laboratory is supported by grant AGL2016-79738-R (AEI/FEDER, EU) from the State Agency for Research (AEI) of Spain, and co-funded by the FEDER Program from the European Union
DS Minerva
RD 28 abr 2026