RT Journal Article
T1 Use of endometrial cytology and metabolic profiles for selection of embryo donor cows
A1 Fernández Sánchez, Francisco Ismael
A1 Barrio López, Mónica
A1 Quintela Arias, Luis Ángel
A1 Becerra González, Juan José
A1 Peña Martínez, Ana Isabel
A1 Martínez Bello, Daniel
A1 García Herradón, Pedro José
A1 Pérez Marín, Carlos C.
K1 Dairy cattle
K1 Embryo transfer
K1 Serum chemistry parameters
K1 Endometritis
K1 Neutrophils
AB The aim of this study was to evaluate the use of endometrial cytology and metabolic profiles for selection of donorcows in embryo transfer programmes. For this purpose, 69 clinically healthy Holstein cows were enrolled in the study.At the start of the superovulation procedure (Day 0), blood and endometrial samples were obtained to determinemetabolic and uterine status, respectively. The cows were then subjected to porcine follicle stimulating hormone (pFSH)superovulation treatment, and embryos were recovered after 7 days. The mean number of embryos obtained per flushwas 9.89 ± 8.21 (4.63 ± 5.34 viable embryos, 0.82 ± 2.01 degenerated embryos and 4.57 ± 6.44 unfertilized ova). Thefollowing statistically significant variables were entered in a regression model: beta-hydroxybutyrate, serum cholesterol,body condition, number of calvings and percentage of neutrophils. In almost all cases, the model explained somepercentage of the variance: total number of embryos, 4.8% (p < 0.05); number of degenerate embryos, 4.2% (p = 0.051);and number of unfertilized ova, 14.2% (p < 0.01). Statistical models for the percentage of viable embryos andunfertilized ova accounted for 24.0% and 29.4% of the variance, respectively, and both were statistically significant(p < 0.01). The model for the percentage of degenerated embryos was statistically significant (p < 0.05) and explained4.4% of the variance. In conclusion, we have demonstrated that positive energy balance and healthy uterus can improveovarian response and the proportion of viable embryos in cows. Efficient tools for monitoring the metabolic and uterinestatus should therefore be used in bovine embryo transfer programmes
PB Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
SN 1695-971X
YR 2014
FD 2014
LK http://hdl.handle.net/10347/22555
UL http://hdl.handle.net/10347/22555
LA eng
NO Fernández Sánchez, F.I., Barrio López, M., Quintela Arias, L.A., Becerra González, J.J., Peña Martínez, A.I., Martínez Bello, D. et al.(2014), Use of endometrial cytology and metabolic profiles for selection of embryo donor cows. Span. J. Agric. Res. vol. 12 (3), 664-671
NO The study was supported by Xunta de Galicia (Programa Sectorial de Medio Rural, Proyecto Ref. PGIDIT07MRU002E) and by FEFRIGA, Santiago deCompostela, Spain
DS Minerva
RD 24 abr 2026