RT Journal Article T1 Assessment of prebiotic potential of Akpan-yoghurt-like product and effects on the human intestinal microbiota A1 Gullón Estévez, Beatriz A1 Pereira, Miguel F. A1 Mestres, Christian A1 Hounhouigan, Joseph A1 Pallet, Dominique A1 Alonso, José Luis A1 Pintado, Manuela K1 Cereal K1 Akpan K1 Prebiotic K1 Functional food K1 FISH AB The effect of Akpan (yoghurt-like product) consumption on the human intestinal microbiota was examined. The prebiotic potential of Akpan was assessed by in vitro fermentation using, as inocula, individual probiotic strains and faecal samples from three healthy donors. In this latter case, the prebiotic activity was assessed through the quantification of short chain fatty acid (SCFA) production, the evolution of the pH and the evaluation of dynamic bacterial population by fluorescent in situ hybridization (FISH). Results demonstrated that Akpan was metabolized after 44 h of fermentation, exerting a prebiotic effect, similar to that observed for fructooligosaccharides (FOS). All the considered bacterial groups significantly increased after Akpan and FOS fermentation. Under the tested conditions, no significant differences between the SCFA concentration produced by Akpan and FOS after of incubation were detected. Our findings contribute to support the utilization of Akpan as a functional food with potential beneficial effects on gastrointestinal health PB Elsevier SN 1756-4646 YR 2015 FD 2015 LK http://hdl.handle.net/10347/24285 UL http://hdl.handle.net/10347/24285 LA eng NO Journal of Functional Foods, Volume 19, Part A, December 2015, Pages 545-553 NO This study was financially supported by the European Seven Framework programme (EU FP7), and is part of the AFTER project (African Food Tradition Revisited by Research) under contract no. 245-025. It was also supported by National Funds from FCT – Fundação para a Ciência e a Tecnologia – through project PEst-OE/EQB/LA0016/2013. B. Gullón is grateful to the Portuguese Foundation for Science and Technology (Fundação para a Ciência e Tecnologia, FCT, Portugal) for funding through the postdoctoral fellowship reference SFRH/BPD/79941/2011 DS Minerva RD 28 abr 2026