RT Journal Article
T1 Multicomponent bioactive extract from red stage Haematococcus pluvialis wet paste: avoiding the drying step and toxic solvents
A1 Castillo Zamora, Aly Jesús
A1 Pereira, Simón
A1 Otero Casal, Ana María
A1 García Jares, Carmen María
A1 Lores Aguín, Marta
K1 Microalgae
K1 GRAS solvents
K1 Carotenoids
K1 Polyphenols
K1 Antioxidants
K1 Matrix solid-phase dispersion (MSPD)
AB This study provides an efficient alternative by extracting bioactive compounds from Haematococcus pluvialis via matrix solid-phase dispersion (MSPD) from its wet form, reducing one of the process steps with the greatest economic impact, the drying of the microalga. To obtain a suitable extract for nutraceutical purposes, solvents with the generally recognised as safe (GRAS) designation (ethanol, ethyl lactate, and ethyl acetate) with limitations of use (acetone) and extractants with higher toxicity such as methanol and methyl-tert-butyl ether (MTBE) are contrasted. Through the optimisation of the extractive process, ethanol, a GRAS solvent, presents the best overall recovery for carotenoid compounds and fatty acids, showing an antioxidant activity of 1.58 mmolTE g−1 DW, comparable to its synthetic alternative of petrochemical origin without the drawback of having limitations in its food use. In addition, the identification of the phenolic compounds, phloroglucinol, p-coumaric acid, gallic acid, and catechin, not previously characterised in red stage H. pluvialis, provides a response to the phenolic activity present in the extract (24.65 mmolGAE g−1 DW). Comparison of the extractive efficiency obtained with the main methods for the extraction of carotenoids and fatty acids in H. pluvialis, in contrast to the proposed method, shows a positive feasibility of this approach
PB Springer
SN 0921-8971
YR 2022
FD 2022
LK http://hdl.handle.net/10347/29197
UL http://hdl.handle.net/10347/29197
LA eng
NO Journal of Applied Phycology 34, 1537–1553 (2022).  https://doi.org/10.1007/s10811-022-02712-3
NO info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/EQC2018-005011-P/ES
NO Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This research was funded by project ED431 2020/06 (Galician Competitive Research Groups Xunta de Galicia). This work was supported by project EQC2018-005011-P (Ministry of Science, Innovation and Universities, Spain). All these programmes are co-funded by FEDER (EU). This article is based upon work from the Sample Preparation Study Group and Network, supported by the Division of Analytical Chemistry of the European Chemical Society
DS Minerva
RD 30 abr 2026