RT Journal Article
T1 Tracing recombinant bovine somatotropin ab(use) through transcriptomics: the potential of bovine somatic cells in a multi-dose longitudinal study
A1 Lamas Freire, Alexandre
A1 Regal López, Patricia
A1 Vázquez Belda, Beatriz Isabel
A1 Miranda López, José Manuel
A1 Cepeda Sáez, Alberto
A1 Franco Abuín, Carlos Manuel
AB In the European Union, the use of recombinant bovine somatotropin (rbST) in dairy cattle is forbidden. Monitoring rbST (ab)use by its direct detection in animal matrices still remains a challenging task. New monitoring methods based on indirect detection of the substance are necessary. A new transcriptomic system based on the use of high-throughput real-time PCR in combination with somatic cells was developed to control rbST administration in dairy animals. A total of nine cows, separated into control and rbST-treated groups, were included in the study. A subcutaneous injection containing 500 mg of rbST was administered to the treated group every 14 days, up to a total of 12 doses. Milk somatic cells (MSCs) were sampled from each animal at different time points throughout 8 months of study. It was possible to obtain the transcriptomic profile of 18 genes in MSCs of rbST-treated and control groups, and using univariate and multivariate statistical analysis control and treated animals were discriminated. The transcription of CCND1, IGF-1R, TNF and IL-1β genes resulted strongly influenced by rbST treatment. The combination of MSCs, transcriptomic tools and statistical analysis has allowed the selection of four genes as potential biomarkers that could be used in a transcriptomic panel for monitoring rbST administration in cows.
PB Nature Publishing Group
YR 2019
FD 2019
LK http://hdl.handle.net/10347/21233
UL http://hdl.handle.net/10347/21233
LA eng
NO Lamas, A., Regal, P., Vázquez, B. et al. Tracing recombinant bovine somatotropin ab(use) through transcriptomics: the potential of bovine somatic cells in a multi-dose longitudinal study. Sci Rep 9, 4788 (2019). https://doi.org/10.1038/s41598-019-41343-6
NO Authors acknowledge the fnancial support of the Spanish Innovation Program “Programa Estatal de Investigación, Desarrollo e Innovación Orientada a los Retos de la Sociedad” (project AGL2014-58881-R)
DS Minerva
RD 26 abr 2026