RT Journal Article
T1 Differential expression of five prosomatostatin genes in the central nervous system of the catshark Scyliorhinus canicula
A1 Sobrido Cameán, Daniel
A1 Tostivint, Hervé
A1 Mazan, Sylvie
A1 Rodicio Rodicio, María Celina
A1 Rodríguez-Moldes Rey, María Isabel
A1 Candal Suárez, Eva María
A1 Anadón Álvarez, Ramón
A1 Barreiro Iglesias, Antón
K1 Brain
K1 Differential gene expression
K1 Elasmobranchs
K1 Shark
K1 Somatostatin precursors
K1 Spinal cord
AB Five prosomatostatin genes (PSST1, PSST2, PSST3, PSST5, and PSST6) have been recently identified in elasmobranchs (Tostivint et al., General and Comparative Endocrinology, 2019, 279, 139–147). In order to gain insight into the contribution of each somatostatin to specific nervous systems circuits and behaviors in this important jawed vertebrate group, we studied the distribution of neurons expressing PSST mRNAs in the central nervous system (CNS) of Scyliorhinus canicula using in situ hybridization. Additionally, we combined in situ hybridization with tyrosine hydroxylase (TH) immunochemistry for better characterization of PSST1 and PSST6 expressing populations. We observed differential expression of PSST1 and PSST6, which are the most widely expressed PSST transcripts, in cell populations of many CNS regions, including the pallium, subpallium, hypothalamus, diencephalon, optic tectum, midbrain tegmentum, and rhombencephalon. Interestingly, numerous small pallial neurons express PSST1 and PSST6, although in different populations judging from the colocalization of TH immunoreactivity and PSST6 expression but not with PSST1. We observed expression of PSST1 in cerebrospinal fluid-contacting (CSF-c) neurons of the hypothalamic paraventricular organ and the central canal of the spinal cord. Unlike PSST1 and PSST6, PSST2, and PSST3 are only expressed in cells of the hypothalamus and in some hindbrain lateral reticular neurons, and PSST5 in cells of the region of the entopeduncular nucleus. Comparative data of brain expression of PSST genes indicate that the somatostatinergic system of sharks is the most complex reported in any fish.
PB Wiley
YR 2020
FD 2020-03-05
LK https://hdl.handle.net/10347/44200
UL https://hdl.handle.net/10347/44200
LA eng
NO Sobrido-Cameán D, Tostivint H, Mazan S, et al. Differential expression of five prosomatostatin genes in the central nervous system of the catshark Scyliorhinus canicula. J Comp Neurol. 2020; 528: 2333–2360. https://doi.org/10.1002/cne.24898
NO This is the peer reviewed version of the following article: Sobrido-Cameán D, Tostivint H, Mazan S, et al. Differential expression of five prosomatostatin genes in the central nervous system of the catshark Scyliorhinus canicula. J Comp Neurol. 2020; 528: 2333–2360, which has been published in final form at https://doi.org/10.1002/cne.24898. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
NO FEDER/Ministerio de Ciencia, Innovación y Universidades - Agencia Estatal de Investigación (Grant numbers: BFU-2017-87079-P to MCR and BFU2017-89861-P to EC and IR-M). Agence Nationale de la Recherche (ANR) grant NEMO no ANR-14-CE02-0020-01 (to HT).
DS Minerva
RD 28 abr 2026