RT Journal Article
T1 Caloric Restriction Prevents Metabolic Dysfunction and the Changes in Hypothalamic Neuropeptides Associated with Obesity Independently of Dietary Fat Content in Rats
A1 Martín, Marina
A1 Rodríguez, Amaia
A1 Gómez Ambrosi, Javier
A1 Ramírez, Beatriz
A1 Becerril, Sara
A1 Catalán, Victoria
A1 López Pérez, Miguel A.
A1 Diéguez González, Carlos
A1 Frühbeck, Gema
A1 Burrell, María Ángela
K1 Food restriction
K1 Gut hormones
K1 Hypothalamic neuropeptides and obesity
AB Energy restriction is a first therapy in the treatment of obesity, but the underlying biological mechanisms have not been completely clarified. We analyzed the effects of restriction of high-fat diet (HFD) on weight loss, circulating gut hormone levels and expression of hypothalamic neuropeptides. Ten-week-old male Wistar rats (n = 40) were randomly distributed into four groups: two fed ad libitum a normal diet (ND) (N group) or a HFD (H group) and two subjected to a 25% caloric restriction of ND (NR group) or HFD (HR group) for 9 weeks. A 25% restriction of HFD over 9 weeks leads to a 36% weight loss with regard to the group fed HFD ad libitum accompanied by normal values in adiposity index and food efficiency ratio (FER). This restriction also carried the normalization of NPY, AgRP and POMC hypothalamic mRNA expression, without changes in CART. Caloric restriction did not succeed in improving glucose homeostasis but reduced HFD-induced hyperinsulinemia. In conclusion, 25% restriction of HFD reduced adiposity and improved metabolism in experimental obesity, without changes in glycemia. Restriction of the HFD triggered the normalization of hypothalamic NPY, AgRP and POMC expression, as well as ghrelin and leptin levels
PB MDPI
YR 2021
FD 2021
LK http://hdl.handle.net/10347/26745
UL http://hdl.handle.net/10347/26745
LA eng
NO Nutrients 2021, 13(7), 2128; https://doi.org/10.3390/nu13072128
NO This work was supported by Ministerio de Economía y Competitividad (SAF2015-71026R) and Fondo de Investigación Sanitaria-FEDER (PI19/00785 and PI19/00990). CIBEROBN is an initiative of the Instituto de Salud Carlos III, Spain
DS Minerva
RD 26 abr 2026