RT Journal Article
T1 Niosomes-based gene delivery systems for effective transfection of human mesenchymal stem cells
A1 Carballo Pedrares, Natalia
A1 Kattar, Axel
A1 Concheiro Nine, Ángel Joaquín
A1 Álvarez Lorenzo, Carmen
A1 Rey Rico, Ana
K1 Niosomes
K1 MSCs
K1 DOTMA
K1 Gene transfer
K1 Sucrose
K1 Stability
AB Gene transfer to mesenchymal stem cells (MSCs) has arisen as a powerful approach to increase the therapeutic potential of this effective cell population. Over recent years, niosomes have emerged as self-assembled carriers with promising performance for gene delivery. The aim of our work was to develop effective niosomes-based DNA delivery platforms for targeting MSCs. Niosomes based on 1,2-di-O-octadecenyl-3-trimethylammonium propane (DOTMA; 0, 7 or 15%) as cationic lipid, cholesterol as helper lipid, and polysorbate 60 as non-ionic surfactant, were prepared using a reverse phase evaporation technique. Niosomes dispersions (filtered or not) and their corresponding nioplexes with a lacZ plasmid were characterized in terms of size, charge, protection, and complexation abilities. DOTMA concentration had a large influence on the physicochemical properties of resulting nioplexes. Transfection efficiency and cytotoxic profiles were assessed in two immortalized cell lines of MSCs. Niosomes formulated with 15% DOTMA provided the highest values of β-galactosidase activity, being similar to those achieved with Lipofectamine®, but showed less cytotoxicity. Filtration of niosomes dispersions before adding to the cells resulted in a loss of their biological activities. Storage of niosomes formulations (for 30 days at room temperature) caused minor modification of their physicochemical properties but also attenuated the transfection capability of the nioplexes. Differently, addition of the lysosomotropic agent sucrose into the culture medium during transfection or to the formulation itself improved the transfection performance of non-filtered niosomes. Indeed, steam heat-sterilized niosomes prepared in sucrose medium demonstrated transfection capability
PB Elsevier
SN 0928-4931
YR 2021
FD 2021
LK http://hdl.handle.net/10347/26721
UL http://hdl.handle.net/10347/26721
LA eng
NO Materials Science and Engineering: C  2021, 128: 112307. https://doi.org/10.1016/j.msec.2021.112307
NO The work was supported by MICINN [RTI2018-099389-A-100; PID2020-113881RB-I00], Agencia Estatal de Investigación (AEI) Spain, Xunta de Galicia [ED431C 2020/17], and FEDER. A. Rey-Rico acknowledges MICINN for the Ramón y Cajal Fellowship [RYC2018-025617-I] and Universidade da Coruña/CISUG for the funding for open access charge. This project was also funded by the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Actions grant agreement N° 813440 (ORBITAL–Ocular Research by Integrated Training And Learning)
DS Minerva
RD 24 abr 2026