RT Journal Article
T1 The Major Storage Protein in Potato Tuber Is Mobilized by a Mechanism Dependent on Its Phosphorylation Status
A1 Bernal Pampín, Javier
A1 Mouzo Calzadilla, Daniel
A1 López-Pedrouso, María
A1 Franco Ruiz, Daniel
A1 García Calvo, Lucio
A1 Zapata Babío, José Carlos
K1 Solanum tuberosum
K1 Patatin
K1 Seed storage proteins
K1 Vegetative storage proteins
K1 Tuber phosphoproteome
K1 Targeted two-dimensional electrophoresis
AB The role of the protein phosphorylation mechanism in the mobilization of vegetative storage proteins (VSPs) is totally unknown. Patatin is the major VSP of the potato (Solanum tuberosum L.) tuber that encompasses multiple differentially phosphorylated isoforms. In this study, temporal changes in the phosphorylation status of patatin isoforms and their involvement in patatin mobilization are investigated using phosphoproteomic methods based on targeted two-dimensional electrophoresis (2-DE). High-resolution 2-DE profiles of patatin isoforms were obtained in four sequential tuber life cycle stages of Kennebec cultivar: endodormancy, bud break, sprouting and plant growth. In-gel multiplex identification of phosphorylated isoforms with Pro-Q Diamond phosphoprotein-specific stain revealed an increase in the number of phosphorylated isoforms after the tuber endodormancy stage. In addition, we found that the phosphorylation status of patatin isoforms significantly changed throughout the tuber life cycle (P < 0.05) using the chemical method of protein dephosphorylation with hydrogen fluoride-pyridine (HF-P) coupled to 2-DE. More specifically, patatin phosphorylation increased by 32% from endodormancy to the tuber sprouting stage and subsequently decreased together with patatin degradation. Patatin isoforms were not randomly mobilized because highly phosphorylated Kuras-isoforms were preferably degraded in comparison to less phosphorylated non-Kuras isoforms. These results lead us to conclude that patatin is mobilized by a mechanism dependent on the phosphorylation status of specific isoforms.
PB MDPI
YR 2019
FD 2019
LK http://hdl.handle.net/10347/21899
UL http://hdl.handle.net/10347/21899
LA eng
NO Bernal, J., Mouzo, D., López-Pedrouso, M., Franco, D., García, L., & Zapata, C. (2019). The Major Storage Protein in Potato Tuber Is Mobilized by a Mechanism Dependent on Its Phosphorylation Status. International Journal of Molecular Sciences, 20(8), 1889. MDPI AG. Retrieved from http://dx.doi.org/10.3390/ijms20081889
NO This research was supported by funds from the Consellería do Medio Rural (Xunta de Galicia, Spain)
DS Minerva
RD 24 abr 2026