RT Journal Article
T1 Reversible Control of Protein Corona Formation on Gold Nanoparticles Using Host–Guest Interactions
A1 Mosquera Mosquera, Jesús
A1 García, Isabel
A1 Henriksen-Lacey, Malou
A1 Martínez-Calvo, Miguel
A1 Dhanjani, Mónica
A1 Mascareñas Cid, José Luis
A1 Liz-Marzán, Luis M.
K1 Cell uptake
K1 Metal nanoparticles
K1 Gold
K1 Colloids
K1 Assays
K1 Protein corona
K1 Gold nanoparticles
K1 Host−guest chemistry
K1 Cellular uptake
K1 Photothermal therapy
AB When nanoparticles (NPs) are exposed to biological media, proteins are adsorbed, forming a so-called protein corona (PC). This cloud of protein aggregates hampers the targeting and transport capabilities of the NPs, thereby compromising their biomedical applications. Therefore, there is a high interest in the development of technologies that allow control over PC formation, as this would provide a handle to manipulate NPs in biological fluids. We present a strategy that enables the reversible disruption of the PC using external stimuli, thereby allowing a precise regulation of NP cellular uptake. The approach, demonstrated for gold nanoparticles (AuNPs), is based on a biorthogonal, supramolecular host–guest interactions between an anionic dye bound to the AuNP surface and a positively charged macromolecular cage. This supramolecular complex effectively behaves as a zwitterionic NP ligand, which is able not only to prevent PC formation but also to disrupt a previously formed hard corona. With this supramolecular stimulus, the cellular internalization of AuNPs can be enhanced by up to 30-fold in some cases, and even NP cellular uptake in phagocytic cells can be regulated. Additionally, we demonstrate that the conditional cell uptake of purposely designed gold nanorods can be used to selectively enhance photothermal cell death
PB ACS Publications
SN 1936-0851
YR 2020
FD 2020
LK http://hdl.handle.net/10347/22786
UL http://hdl.handle.net/10347/22786
LA eng
NO ACS Nano 2020, 14, 5, 5382–5391
NO Funding was received from MINECO (SAF2016-76689-R, MAT2017-86659-R), Xunta de Galicia (2015-CP082, ED431C 2017/19, Centro singular de investigación de Galicia accreditation 2019-2022, ED431G 2019/03), the European Union (European Regional Development Fund), and the European Research Council (ERC AdG No. 787510 to L.M.L.-M.; ERC AdG No. 340055 to J.L.M). J.M. and M.M.-C. thank MINECO for Juan de la Cierva fellowships (FJCI-2015-25080 and IJCI-2014-19326). The proteomic analysis was performed in the proteomics platform at CIC bioGUNE, which is supported by Basque Department of Industry, Tourism and Trade (Etortek and Elkartek programs), the Innovation Technology Department of the Bizkaia County; The ProteoRed-ISCIII (Grant No. PRB3 IPT17/0019); CIBERehd Network, and Severo Ochoa Grant (No. SEV-2016-0644). This work was performed under the Maria de Maeztu Units of Excellence Program from the Spanish State Research Agency, Grant No. MDM-2017-0720
DS Minerva
RD 28 abr 2026