RT Journal Article
T1 Production of transglutaminase by streptoverticillium ladakanum NRRL-3191 using glycerol as carbon source
T2 Proizvodnja transglutaminaze iz Streptoverticillium ladakanum NRRL-3191 uz glicerol kao izvor ugljika
A1 Téllez Luis, Simón J.
A1 Ramírez de León, José Alberto
A1 Vázquez Vázquez, Manuel
K1 Transglutaminase
K1 Glycerol
K1 Casein
K1 Streptoverticillium ladakanum
AB The enzyme transglutaminase (TG) catalyses the formation of covalent bonds betweenadjacent proteins, thereby improving the gel structure of proteins and has important applications for the food industry. The aims of this work were: (i) to elucidate the effect ofagitation speed during the biotechnological production of TG by Streptoverticillium ladakanum NRRL-3191 using glycerol as carbon source; and (ii) to improve TG production byoptimising the composition of media based on glycerol, xylose and casein. An agitationspeed of 250 rpm and a fermentation time of 72 h resulted in the optimal enzymatic activity(0.628 U/mL) with a productivity of 0.087 U/(mL·h). The composition of media with glycerol, xylose and casein were optimised using an experimental design to improve TG production. The model predicts that the maximum TG activity (0.725 U/mL) can be obtainedusing glycerol 50.5 g/L and casein 20 g/L without the addition of xylose
PB University of Zagreb
SN 1330-9862
YR 2004
FD 2004
LK http://hdl.handle.net/10347/23056
UL http://hdl.handle.net/10347/23056
LA eng
NO Téllez Luis, Simón J. ; Ramírez, José A. ; Vázquez, Manuel. (2004). Production of Transglutaminase by S. ladakanum NRRL-3191 Using Glycerol as Carbon Source. "Food Technol. Biotechnol." vol. 42, n. 2, p. 75–81
NO A grant from the PROMEP program of the Secretaríade Educación Pública (México) to author Téllez-Luis isgratefully acknowledged
DS Minerva
RD 1 may 2026