RT Journal Article
T1 Evaluation of the Novel mTA10 Selective Broth, MSB, for the Co-Enrichment and Detection of Salmonella spp., Escherichia coli O157 and Listeria monocytogenes in Ready-to-Eat Salad Samples
A1 Costa Ribeiro, Ana
A1 Lamas Freire, Alexandre
A1 Prado Rodríguez, Marta
A1 Garrido Maestu, Alejandro
K1 selective enrichment
K1 multiplex qPCR
K1 Salmonella spp.
K1 Escherichia coli O157
K1 Listeria monocytogenes
AB Multiplex assays implementing DNA-based methods have been demonstrated as suitable alternatives to culture-based microbiological methods; however, in most cases, they still require a suitable enrichment step. Finding suitable enrichment conditions for different bacteria may result in challenges. In the present study, a novel selective broth named MSB (mTA10 selective broth) was formulated for the simultaneous recovery of Salmonella spp., E. coli O157:H7 and L. monocytogenes. Attention was paid to ensure the optimal enrichment of L. monocytogenes as its enrichment is more challenging. To this end, cellobiose was added to increase the growth of L. monocytogenes, and sodium pyruvate was also added to improve the recovery of stressed bacteria. Four selective agents were added, namely nalidixic acid, sodium cholate, lithium chloride and potassium tellurite, to control the growth of interfering microorganisms. It was concluded that the novel broth was suitable for the simultaneous enrichment of the target pathogens, allowing them to reach concentrations higher than 7 log CFU/mL for each bacterium in pure culture. Furthermore, all heavily contaminated ready-to-eat salad samples reached concentrations higher than 5 log CFU/g. Finally, after 24 h of enrichment of spiked salad, it was possible to detect concentrations below 10 CFU/25 g.
PB MDPI
SN 2304-8158
YR 2023
FD 2023-12-23
LK https://hdl.handle.net/10347/43530
UL https://hdl.handle.net/10347/43530
LA eng
NO Costa-Ribeiro, A.; Lamas, A.; Prado, M.; Garrido-Maestu, A. Evaluation of the Novel mTA10 Selective Broth, MSB, for the Co-Enrichment and Detection of Salmonella spp., Escherichia coli O157 and Listeria monocytogenes in Ready-to-Eat Salad Samples. Foods 2024, 13, 63. https://doi.org/ 10.3390/foods13010063
NO Fundação para a Ciência e a Tecnologia under the Scientific Employment Stimulus Program (2021.02810.CEECIND)
NO Agenda SMARTgNOSTICS–Global Testing & Diagnostics Solutions for antimicrobial resistances”, with reference n.º C644915155-00000024, co-funded by Component C5–Capitalisation and Business Innovation under the Portuguese Resilience and Recovery Plan, through the NextGenerationEU Fund.
DS Minerva
RD 24 abr 2026