Development of a transcriptomic protocol combining bovine somatic cells and OpenArray® technology to trace the ab(use) of somatotropin in dairy cattle

Abstract

In the case of recombinant bovine somatotropin (rbST), in Europe it is key to control misuse in cattle in vivo, during the period of lactation of the cow. Therefore, target samples have to be easy to collect, the method of collection should be non-invasive and it has to be economically viable. The goal of this study was to develop a transcriptomic protocol based in the combination of High-Throughput Real-Time PCR and milk somatic cells to detect the ab(use) of rbST in dairy cattle. The results showed that MSCs are an optimal alternative to isolate RNA with good quality using a simple protocol for their collection. The use of OpenArray® technology made possible to establish a transcriptomic profile of treated and control cows that could be used to detect the illegal use of rBST in dairy cattle in EU