Genetic Targeting of GRP78 in the VMH Improves Obesity Independently of Food Intake

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dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicasgl
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Fisioloxíagl
dc.contributor.authorLiñares Pose, Laura
dc.contributor.authorRial Pensado, Eva
dc.contributor.authorEstévez Salguero, Ánxela
dc.contributor.authorMilbank, Edward
dc.contributor.authorGonzález García, Ismael
dc.contributor.authorRodríguez, Claudia
dc.contributor.authorSeoane Collazo, Patricia
dc.contributor.authorMartínez Sánchez, Noelia
dc.contributor.authorNogueiras Pozo, Rubén
dc.contributor.authorPrieto, Dolores
dc.contributor.authorDiéguez González, Carlos
dc.contributor.authorContreras, Cristina
dc.contributor.authorLópez Pérez, Miguel A.
dc.date.accessioned2020-05-06T15:09:34Z
dc.date.available2020-05-06T15:09:34Z
dc.date.issued2018
dc.description.abstractRecent data have demonstrated that the hypothalamic GRP78/BiP (glucose regulated protein 78 kDa/binding immunoglobulin protein) modulates brown adipose tissue (BAT) thermogenesis by acting downstream on AMP-activated protein kinase (AMPK). Herein, we aimed to investigate whether genetic over-expression of GRP78 in the ventromedial nucleus of the hypothalamus (VMH: a key site regulating thermogenesis) could ameliorate very high fat diet (vHFD)-induced obesity. Our data showed that stereotaxic treatment with adenoviruses harboring GRP78 in the VMH reduced hypothalamic endoplasmic reticulum ER stress and reversed vHFD-induced obesity. Herein, we also demonstrated that this body weight decrease was more likely associated with an increased BAT thermogenesis and browning of white adipose tissue (WAT) than to anorexia. Overall, these results indicate that the modulation of GRP78 in the VMH may be a target against obesitygl
dc.description.peerreviewedSIgl
dc.description.sponsorshipThe research leading to these results has received funding from Xunta de Galicia (R.N.: 2015-CP080 and 2016-PG057; M.L.: 2015-CP079), MINECO co-funded by the FEDER Program of EU (R.N.: BFU2015-70664R; D.P.: SAF2016-77526-R; C.D.: BFU2017-87721; M.L.: SAF2015-71026-R and BFU2015-70454-REDT/Adipoplast). The CiMUS is supported by the Xunta de Galicia (2016-2019, ED431G/05). L.L.-P. is a recipient of a fellowship from Xunta de Galicia (ED481A-2016/094); E.R.-P. is a recipient of a fellowship from MINECO (BES-2015-072743); A.E.-S. is a recipient of a fellowship from MINECO (FPI/BES-2016-077439); C.R. is a recipient of a fellowship from MINECO (FPU16/04582). CIBER Fisiopatología de la Obesidad y Nutrición is an initiative of ISCIIIgl
dc.identifier.citationLiñares-Pose, L.; Rial-Pensado, E.; Estévez-Salguero, Á.; Milbank, E.; González-García, I.; Rodríguez, C.; Seoane-Collazo, P.; Martinez-Sánchez, N.; Nogueiras, R.; Prieto, D.; Diéguez, C.; Contreras, C.; López, M. Genetic Targeting of GRP78 in the VMH Improves Obesity Independently of Food Intake. Genes 2018, 9, 357gl
dc.identifier.doi10.3390/genes9070357
dc.identifier.essn2073-4425
dc.identifier.urihttp://hdl.handle.net/10347/22089
dc.language.isoenggl
dc.publisherMDPIgl
dc.relation.publisherversionhttps://doi.org/10.3390/genes9070357gl
dc.rights© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)gl
dc.rights.accessRightsopen accessgl
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBrown adipose tissuegl
dc.subjectBrowninggl
dc.subjectER stressgl
dc.subjectGRP78gl
dc.subjectHypothalamusgl
dc.subjectThermogenesisgl
dc.subjectWhite adipose tissuestressgl
dc.subjectGRP78gl
dc.subjectHypothalamusgl
dc.subjectThermogenesisgl
dc.subjectWhite adipose tissuegl
dc.titleGenetic Targeting of GRP78 in the VMH Improves Obesity Independently of Food Intakegl
dc.typejournal articlegl
dc.type.hasVersionVoRgl
dspace.entity.typePublication
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