Microsatellite markers for the molecular characterisation of Neospora caninum: application to clinical samples

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dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Anatomía, Produción Animal e Ciencias Clínicas Veterinarias
dc.contributor.authorPedraza-Díaz, Susana
dc.contributor.authorMarugán Hernández, Virginia
dc.contributor.authorCollantes Fernández, Esther
dc.contributor.authorRegidor Cerrillo, Javier
dc.contributor.authorRojo Montejo, Silvia
dc.contributor.authorGómez Bautista, Mercedes
dc.contributor.authorOrtega Mora, Luis Miguel
dc.date.accessioned2025-02-03T09:36:51Z
dc.date.available2025-02-03T09:36:51Z
dc.date.issued2009
dc.description.abstractWe report here on the development of a nested PCR procedure for the application of Neospora caninum microsatellite markers to clinical samples. Genotyping technology by fluorescently labelled DNA fragment analysis was used in combination with DNA sequencing for those markers which show additional SNPs or complex repetitive sequences. Twenty-nine DNA samples from naturally infected bovine aborted foetuses from two regions of Spain where N. caninum had been detected by nested PCR of the ITS1 rRNA region were analysed using these microsatellites. Complete, or almost complete allele profiles were obtained from 18 samples. Two pairs of DNA samples showed identical profiles, these originated from twins and foetuses from the same herd, respectively. The multilocus analysis performed showed sub clustering of isolates according to their geographical origin. These results highlight the usefulness of these markers for the molecular characterization of isolates of N. caninum and for isolate tracking in live vaccine development.
dc.description.peerreviewedNON
dc.description.sponsorshipThis work was partially funded by I.N.I.A. (RTA04-047-C2) and the Spanish Ministry of Education and Science (AGL2007-60132/GAN). V. Marugán-Hernández and J. Regidor-Cerrillo were funded by the Spanish Ministry of Education and Science and by Consejería de Educación (Comunidad de Madrid), respectively. This work was part of the E.U. research collaboration COST Action-857.
dc.identifier.citationVeterinary Parasitology Volume 166, Issues 1–2, 3 December 2009, Pages 38-46
dc.identifier.doi10.1016/j.vetpar.2009.07.043
dc.identifier.urihttps://hdl.handle.net/10347/39495
dc.journal.titleVeterinary Parasitology
dc.language.isoeng
dc.page.final46
dc.page.initial38
dc.publisherElsevier
dc.relation.projectIDinfo:eu-repo/grantAgreement/MEC//AGL2007-60132/ES/DESARROLLO DE VACUNAS FRENTE A LA NEOSPOROSIS BOVINA: IDENTIFICACION DE NUEVAS DIANAS VACUNALES Y MEJORA DE LOS SISTEMAS DE ADYUVANTACION/
dc.relation.publisherversionhttps://doi.org/10.1016/j.vetpar.2009.07.043
dc.rights.accessRightsopen access
dc.subjectNeospora caninum
dc.subjectBovine aborted foetuses
dc.subjectMicrosatellite markers
dc.subjectNested PCR
dc.subject.classificationInvestigación
dc.titleMicrosatellite markers for the molecular characterisation of Neospora caninum: application to clinical samples
dc.typejournal article
dc.type.hasVersionAO
dc.volume.number166
dspace.entity.typePublication
relation.isAuthorOfPublication21764f77-8250-4244-97f9-e0e6a27ee340
relation.isAuthorOfPublication.latestForDiscovery21764f77-8250-4244-97f9-e0e6a27ee340

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