A Single Mutation is Sufficient to Modify the Metal Selectivity and Specificity of a Eukaryotic Manganese Superoxide Dismutase to Encompass Iron

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dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Molecularesgl
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Química Orgánicagl
dc.contributor.authorHunter, Thérèse
dc.contributor.authorBonetta, Rosalin
dc.contributor.authorSacco, Anthony
dc.contributor.authorVella, Marita
dc.contributor.authorSultana, Paul-Michael
dc.contributor.authorTrinh, Chi H.
dc.contributor.authorFadia, Hava B. R.
dc.contributor.authorBorowski, Tomasz
dc.contributor.authorGarcía Fandiño, Rebeca
dc.contributor.authorStockner, Thomas
dc.contributor.authorHunter, Gary J.
dc.date.accessioned2021-01-26T07:58:26Z
dc.date.available2021-01-26T07:58:26Z
dc.date.issued2018
dc.description.abstractWe have generated a site‐directed mutant of the manganese superoxide dismutase SOD‐3 of C.elegans (MnSOD‐3) which modifies the metal specificity of the enzyme. While wild‐type MnSOD‐3 functions with manganese in the active site (3600 U mg−1 of protein) it has little or no activity when iron is incorporated. However, when histidine replaces glutamine 142 in the active site, the enzyme retains 50 % of its activity and becomes cambialistic for its metal cofactor exhibiting very similar specific activity with either manganese or irongl
dc.description.peerreviewedSIgl
dc.description.sponsorshipWork reported here was carried out using funding from UoM grants PHBIN02‐1, PHBRP03‐05 and PHBRP03‐17. The European Union Erasmus+ programme (project number: 2016‐1‐PL01‐KA103‐023786) is acknowledged for providing financial support for the mobility traineeship of T.B. This research was supported in part by PL‐Grid Infrastructure. We wish to thank the EU COST association for support through COST actions CM1305 and CM1306gl
dc.identifier.citationT. Hunter, R. Bonetta, A. Sacco, M. Vella, P.-M. Sultana, C. H. Trinh, H. B. R. Fadia, T. Borowski, R. Garcia-Fandiño, T. Stockner, G. J. Hunter, Chem. Eur. J. 2018, 24, 5303gl
dc.identifier.doi10.1002/chem.201704655
dc.identifier.essn1521-3765
dc.identifier.urihttp://hdl.handle.net/10347/24312
dc.language.isoenggl
dc.publisherWileygl
dc.relation.publisherversionhttps://doi.org/10.1002/chem.201704655gl
dc.rights© 2018 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are madegl
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.accessRightsopen accessgl
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectEnzymesgl
dc.subjectIrongl
dc.subjectManganesegl
dc.subjectMetalloproteingl
dc.subjectSuperoxide dismutasegl
dc.titleA Single Mutation is Sufficient to Modify the Metal Selectivity and Specificity of a Eukaryotic Manganese Superoxide Dismutase to Encompass Irongl
dc.typejournal articlegl
dc.type.hasVersionVoRgl
dspace.entity.typePublication
relation.isAuthorOfPublication7207f196-ba01-47c3-a5a7-dac268e007d3
relation.isAuthorOfPublication.latestForDiscovery7207f196-ba01-47c3-a5a7-dac268e007d3

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