In situ detection of the protein corona in complex environments

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2017_natcomm_carril_in_situ.pdf (950.61 KB)
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URI: http://hdl.handle.net/10347/19758
E-ISSN: 2041-1723
DOI: 10.1038/s41467-017-01826-4

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2017

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Springer Nature
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Abstract

Colloidal nanoparticles (NPs) are a versatile potential platform for in vivo nanomedicine. Inside blood circulation, NPs may undergo drastic changes, such as by formation of a protein corona. The in vivo corona cannot be completely emulated by the corona formed in blood. Thus, in situ detection in complex media, and ultimately in vivo, is required. Here we present a methodology for determining protein corona formation in complex media. NPs are labeled with 19F and their diffusion coefficient measured using 19F diffusion-ordered nuclear magnetic resonance (NMR) spectroscopy. 19F diffusion NMR measurements of hydrodynamic radii allow for in situ characterization of NPs in complex environments by quantification of protein adsorption to the surface of NPs, as determined by increase in hydrodynamic radius. The methodology is not optics based, and thus can be used in turbid environments, as in the presence of cells

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Biophysical chemistry| Nanoparticles| NMR spectroscopy| Surface chemistry

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Carril, M., Padro, D., del Pino, P., Carrillo-Carrion, C., Gallego, M., & Parak, W. (2017). In situ detection of the protein corona in complex environments. Nature Communications, 8(1). doi: 10.1038/s41467-017-01826-4

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This work was supported by the German Research Foundation (DFG grant DFG Grant PA 794/25-1 to W.J.P.). Parts of this work were funded by MINECO (CTQ2015-68413-R to M.C.). M.C. acknowledges Ikerbasque for a Research Fellow position. C.C.-C. acknowledges MINECO for a Juan de la Cierva—Incorporación contract. P.d.P. acknowledges financial support from MINECO (RYC-2014–16962), the Xunta de Galicia (Centro singular de investigación de Galicia accreditation 2016–2019, ED431G/09), and the European Union (European Regional Development Fund—ERDF).

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© The Author(s) 2017. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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Centro de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS)
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