An Optimized Immunoprecipitation Protocol for Assessing Protein-RNA Interactions In Vitro

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dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas (CiMUS)
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Bioquímica e Bioloxía Molecular
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Fisioloxía
dc.contributor.authorFuentes Iglesias, Alejandro
dc.contributor.authorGarcía Outeiral, Vera
dc.contributor.authorPardavila Paz, José Ángel
dc.contributor.authorWang, Jianlong
dc.contributor.authorFidalgo Pérez, Miguel Ángel
dc.contributor.authorGuallar Artal, Diana
dc.date.accessioned2025-01-17T09:03:07Z
dc.date.available2025-01-17T09:03:07Z
dc.date.issued2020-09-04
dc.description.abstractRNA-binding proteins are key regulators of cell identity and function, which underscores the need for unbiased and versatile protocols to identify and characterize novel protein-RNA interactions. Here, we describe a simple and cost-effective in vitro RNA immunoprecipitation (iv-RIP) method to assess the direct or indirect RNA-binding ability of any protein of interest. The versatility of this method relies on the adaptability of the immunoprecipitation conditions and the choice of the RNA, which exponentially broadens the range of potential applications
dc.description.peerreviewedSI
dc.identifier.citationFuentes-Iglesias et al., An Optimized Immunoprecipitation Protocol for Assessing Protein-RNA Interactions In Vitro, STAR Protocols (2020), https://doi.org/10.1016/j.xpro.2020.100093
dc.identifier.doi10.1016/j.xpro.2020.100093
dc.identifier.essn2666-1667
dc.identifier.urihttps://hdl.handle.net/10347/38657
dc.issue.number2
dc.journal.titleStar Protocols
dc.language.isoeng
dc.page.final23
dc.page.initial1
dc.publisherCell Press
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105739GB-100
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096708-J-I00/ES/IMPACTO DE LA MODIFICACION HM5C DEL ARN EN EL ENVEJECIMIENTO Y EL REJUVENECIMIENTO/
dc.relation.publisherversionhttps://doi.org/10.1016/j.xpro.2020.100093
dc.rights© 2020 The Author(s). This is an open access article under the CC BY-NC-ND license
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectImmunoprecipitation Protocol
dc.subjectProtein-RNA Interactions
dc.subjectRNA-binding proteins
dc.subjectin vitro RNA immunoprecipitation
dc.subjectiv-RIP
dc.subject.classification32 Ciencias médicas
dc.titleAn Optimized Immunoprecipitation Protocol for Assessing Protein-RNA Interactions In Vitro
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number1
dspace.entity.typePublication
relation.isAuthorOfPublicationc2e8c7dd-5b10-4a18-916d-eed1c5831aff
relation.isAuthorOfPublicationd94656ad-ed33-4a6c-9407-fc1d84b41289
relation.isAuthorOfPublication.latestForDiscoveryc2e8c7dd-5b10-4a18-916d-eed1c5831aff

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