New insights on the degradation of polystyrene and polypropylene by larvae of the superworm Zophobas atratus and gut bacterial consortium enrichments obtained under different culture conditions

Abstract

This study aims to deepen knowledge of the biodegradation of plastics, focusing on polypropylene (PP) fabric from surgical masks and polystyrene (PS) by larvae of Zophobas atratus as well as of specialized bacterial consortia from their gut, which were obtained in different enrichment conditions (aerobic, anaerobic, presence or absence of combined nitrogen). Plastics ingested by larvae obtained in Spain did not show any signs of oxidation but only limited depolymerization, preferably from the lowest molecular weight chains. Gut microbiota composition changed as an effect of plastic feeding. Such differences were more evident in bacterial enrichment cultures, where the polymer type influenced the composition more than by culture conditions, with an increase in the presence of nitrogen-fixers in anaerobic conditions. PS and PP degradation by different enrichment cultures was confirmed under aerobic and anaerobic conditions by respirometry tests, with anaerobic conditions favouring a more active plastic degradation. In addition, exposure to selected bacterial consortia in aerobiosis induced limited surface oxidation of PS. This possibly indicates that different biochemical routes are being utilized in the anaerobic gut and in aerobic conditions to degrade the polymer.