High-affinity A/T-rich DNA binding with a dimeric bisbenzamidine

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dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS)
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Bioquímica e Bioloxía Molecular
dc.contributor.authorBouzada Reboredo, David
dc.contributor.authorPaul, Ananya
dc.contributor.authorSánchez López, Mateo Isidro
dc.contributor.authorDomínguez-Asenjo, Bárbara
dc.contributor.authorLence Quintana, Emilio José
dc.contributor.authorMelle-Franco, Manuel
dc.contributor.authorArdá, Ana
dc.contributor.authorBarreiro Piñeiro, Natalia
dc.contributor.authorPenas, Cristina
dc.contributor.authorGómez González, Jacobo
dc.contributor.authorJiménez-Barbero, Jesús
dc.contributor.authorMoreno, Javier
dc.contributor.authorGonzález Bello, Concepción
dc.contributor.authorWilson, W. David
dc.contributor.authorMascareñas Cid, José Luis
dc.contributor.authorVázquez Sentís, Marco Eugenio
dc.date.accessioned2025-03-07T07:26:04Z
dc.date.available2025-03-07T07:26:04Z
dc.date.issued2024
dc.description.abstractA bisbenzamidine DNA binding agent can be easily dimerized by alkylation of its terminal amidine groups to afford an extended minor groove binder with over 20-fold enhanced DNA affinity toward extended A/T-rich sites. Nuclear magnetic resonance experiments in combination with molecular dynamics simulation studies provide structural insight into the insertion of this compound in the DNA minor groove, and antimicrobial assays demonstrate that the increased affinity translates into higher antileishmanial activity.
dc.description.peerreviewedSI
dc.description.sponsorshipAgencia Estatal de Investigación [RTI2018-099877-B-I00, PID2022-136963OB-I00, PID2021-127702NB-I00]; Xunta de Galicia [ED431C 2021/29, ED431G 2023/03]; European Regional Development Fund; CICECO [IF/00894/2015, UIDB/50011/2020, UIDP/50011/2020, LA/P/0006/2020]; EDCTP [RIA2020S-3301]; ISCIII [PI21CIII/00005]; CIBERINFEC [CB21/13/00018].
dc.identifier.citationBouzada, D., Paul, A., Sánchez, M. I., Domínguez-Asenjo, B., Lence, E., Melle-Franco, M., Ardá, A., Barreiro-Piñeiro, N., Penas, C., Gómez-González, J., Jiménez-Barbero, J., Moreno, J., González-Bello, C., Wilson, W. D., Mascareñas, J. L., & Vázquez, M. E. (2024). High-affinity A/T-rich DNA binding with a dimeric bisbenzamidine. NAR Molecular Medicine, 1(4), Article ugae022 . https://doi.org/10.1093/narmme/ugae022
dc.identifier.doidoi.org/10.1093/narmme/ugae022
dc.identifier.essn2976-856X
dc.identifier.issn2976-856X
dc.identifier.urihttps://hdl.handle.net/10347/40115
dc.issue.number4
dc.journal.titleNAR Molecular Medicine
dc.language.isoeng
dc.publisherOxford Academic
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099877-B-I00/ES/PLATAFORMAS PEPTIDICAS PARA APLICACIONES EN QUIMICA (BIO)SUPRAMOLECULAR Y DE MATERIALES, CATALISIS Y SENSORES (SWISSKNIFE)/
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-136963OB-I00/ES/NUEVOS AGENTES ANTIBACTERIANOS DE PRECISION Y TERAPIAS COMBINADAS PARA COMBATIR INFECCIONES MULTIRRESISTENTES/
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-127702NB-I00/ES/HERRAMIENTAS PEPTIDICAS EN SENSORES, CATALISIS Y CIENCIA DE MATERIALES/
dc.relation.publisherversionhttps://doi.org/10.1093/narmme/ugae022
dc.rightsAttribution-NonCommercial 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectDimeric bisbenzamidine
dc.subjectADN
dc.subjectA/T
dc.subject.classification2306 Química orgánica
dc.titleHigh-affinity A/T-rich DNA binding with a dimeric bisbenzamidine
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number1
dspace.entity.typePublication
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