Optimising medium chain carboxylate production in xylan mixed-culture monofermentation

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dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Enxeñaría Química
dc.contributor.authorIglesias Riobó, Juan
dc.contributor.authorBonatelli, M. L.
dc.contributor.authorMachado Fernández, C.
dc.contributor.authorMauricio Iglesias, Miguel
dc.contributor.authorCarballa Arcos, Marta
dc.date.accessioned2025-02-06T11:42:15Z
dc.date.available2025-02-06T11:42:15Z
dc.date.issued2025-03
dc.description.abstractThis work investigates the optimization of medium-chain carboxylate (MCC) production through xylan mixed-culture monofermentation. The pH screening in batch assays showed that the hydrolysis stage and selectivity towards MCC precursors were optimised at pH 6. Subsequently, a continuous stirred tank reactor (CSTR) and a Sequential Batch Reactor (SBR) were operated at different Hydraulic Retention Times (HRT), revealing that the SBR at HRT 2 days yielded the highest caproic acid since lactic acid availability and chain elongation process were balanced. An enriched medium with yeast extract and vitamins favoured the growth of chain elongators, and therefore, the MCC production. Moreover, cross-feeding interaction between bacteria in xylan fermentation was observed, and Pseudoramibacter was present in the highest caproic acid yields. This work highlights the impact of selecting the proper operational window to optimise one-stage MCC production.
dc.description.sponsorshipThis research was funded by Spanish Government through the Cell4Chem project (PCI2021-121989, ERACoBioTech 3rd call). The authors J. I.-R., M. M.-I., M. C. belong to a Galician Competitive Research Group (ED431C-2021/37). M. L. B. work was carried out as part of the European project Cell4Chem (German funding code: 100572058) that is co-financed by means of taxation based on the budget adopted by the representatives of the Landtag of Saxony.
dc.identifier.citationBioresource Technology Volume 420, March 2025, 132124
dc.identifier.doi10.1016/j.biortech.2025.132124
dc.identifier.issn1873-2976
dc.identifier.urihttps://hdl.handle.net/10347/39600
dc.journal.titleBioresource Technology
dc.language.isoeng
dc.publisherElsevier
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PCI2021-121989/ES/ENGINEERING MICROBIAL COMMUNITIES FOR THE CONVERSION OF LIGNOCELLULOSE INTO MEDIUM-CHAIN CARBOXYLATES/
dc.relation.publisherversionhttps://doi.org/10.1016/j.biortech.2025.132124
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsembargoed access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectChain elongation
dc.subjectHemicellulose
dc.subjectCaproate
dc.subjectVitamins
dc.subjectYeast extract
dc.titleOptimising medium chain carboxylate production in xylan mixed-culture monofermentation
dc.typejournal article
dc.type.hasVersionAM
dc.volume.number420
dspace.entity.typePublication
relation.isAuthorOfPublicationb098e7de-f49e-4335-9f8d-d70a445f4a69
relation.isAuthorOfPublicationf574e8ce-1a88-4045-bc74-d48db358fc70
relation.isAuthorOfPublication.latestForDiscoveryb098e7de-f49e-4335-9f8d-d70a445f4a69

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