A serum cytotoxicity assay for measuring the resistance of turbot to the ciliate Philasterides dicentrarchi

Simple item page
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Bioloxía Funcional
dc.contributor.affiliationUniversidade de Santiago de Compostela. Instituto de Investigación do Medio Acuático para Unha Saúde Global (iARCUS)
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Bioloxía Funcional
dc.contributor.authorGulías Otero, Paola
dc.contributor.authorSueiro Benavides, Rosa Ana
dc.contributor.authorGonzález Barreiro, Óscar
dc.contributor.authorBrea Portela, Cristina
dc.contributor.authorMacías Sancho, Joaquín
dc.contributor.authorLeiro Vidal, José Manuel
dc.contributor.authorLamas Fernández, Jesús
dc.date.accessioned2025-12-15T13:01:42Z
dc.date.available2025-12-15T13:01:42Z
dc.date.issued2025-10-30
dc.description.abstractIn previous studies we have found that the complement system plays an essential role in the resistance of fish to infection by the ciliate parasite Philasterides dicentrarchi. The present study shows that it is possible to predict resistance to P. dicentrarchi in the turbot Scophthalmus maximus by using a complement-dependent serum cytotoxicity assay. Fish were immunised against P. dicentrarchi, and fish mortality following experimental challenge, specific serum antibody levels and serum cytotoxicity were compared. We found a correlation between the results obtained regarding the killing activity of serum and the protection generated in turbot by the vaccine after experimental challenge. The study findings suggest that the serum cytotoxicity assay can be used to assess the protection generated by the vaccine against P. dicentrarchi in turbot, thereby reducing both the number of fish required for the experiments and the pain and distress associated with experimental infection
dc.description.peerreviewedSI
dc.description.sponsorshipIDI-20200702 from the Ministerio de Economía, Industria y Competitividad
dc.description.sponsorship2021-PG042 from the Xunta de Galicia (Spain)
dc.description.sponsorshipPrograma de Ciencias Mariñas de Galicia (2022-CP084)
dc.identifier.citationGuliás P, Sueiro RA, González O, Brea C, Macías J, Leiro JM, Lamas J. (2025). A serum cytotoxicity assay for measuring the resistance of turbot to the ciliate Philasterides dicentrarchi. Comparative Immunology Reports 9 (2025), 200259. doi: https://doi.org/10.1016/j.cirep.2025.200259
dc.identifier.doi10.1016/j.cirep.2025.200259
dc.identifier.issn2950-3116
dc.identifier.urihttps://hdl.handle.net/10347/44491
dc.issue.number200259
dc.journal.titleComparative Immunology Reports
dc.language.isoeng
dc.page.final7
dc.page.initial1
dc.publisherElsevier
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020–113087RB-I00
dc.relation.publisherversionhttps://doi.org/10.1016/j.cirep.2025.200259
dc.rights© 2025 The Authors. Published by Elsevier Ltd. This article is available under the Creative Commons CC-BY-NC-ND license
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectPhilasterides dicentrarchi
dc.subjectFish
dc.subjectComplement system
dc.subjectCytotoxicity
dc.subjectImmunization
dc.subjectDisease resistance
dc.subject.classificationInvestigación
dc.titleA serum cytotoxicity assay for measuring the resistance of turbot to the ciliate Philasterides dicentrarchi
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number9
dspace.entity.typePublication
relation.isAuthorOfPublication18414ba7-710a-4d0f-bf71-a494fc048c9b
relation.isAuthorOfPublication1405b8aa-a3ef-4048-938e-e53820a309b5
relation.isAuthorOfPublication.latestForDiscovery18414ba7-710a-4d0f-bf71-a494fc048c9b

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
2025_CompImmRep_Gulias_Serum.pdf
Size:
1.74 MB
Format:
Adobe Portable Document Format
Download

Collections

Bioloxía Funcional
Instituto de Investigación do Medio Acuático para unha Saúde Global (iARCUS)
Microbioloxía e Parasitoloxía